دورية أكاديمية
أعرض في EDS

Improved production of L-threonine in Escherichia coli by use of a DNA scaffold system.

التفاصيل البيبلوغرافية
العنوان: Improved production of L-threonine in Escherichia coli by use of a DNA scaffold system.
المؤلفون: Lee JH; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea., Jung SC, Bui le M, Kang KH, Song JJ, Kim SC
المصدر: Applied and environmental microbiology [Appl Environ Microbiol] 2013 Feb; Vol. 79 (3), pp. 774-82. Date of Electronic Publication: 2012 Nov 16.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5336 (Electronic) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, American Society for Microbiology.
مواضيع طبية MeSH: Metabolic Engineering*, Biosynthetic Pathways/*genetics , Escherichia coli/*enzymology , Escherichia coli/*metabolism , Threonine/*biosynthesis, DNA/metabolism ; Escherichia coli/genetics ; Macromolecular Substances ; Multienzyme Complexes ; Protein Binding ; Zinc Fingers
مستخلص: Despite numerous approaches for the development of l-threonine-producing strains, strain development is still hampered by the intrinsic inefficiency of metabolic reactions caused by simple diffusion and random collisions of enzymes and metabolites. A scaffold system, which can promote the proximity of metabolic enzymes and increase the local concentration of intermediates, was reported to be one of the most promising solutions. Here, we report an improvement in l-threonine production in Escherichia coli using a DNA scaffold system, in which a zinc finger protein serves as an adapter for the site-specific binding of each enzyme involved in l-threonine production to a precisely ordered location on a DNA double helix to increase the proximity of enzymes and the local concentration of metabolites to maximize production. The optimized DNA scaffold system for l-threonine production significantly increased the efficiency of the threonine biosynthetic pathway in E. coli, substantially reducing the production time for l-threonine (by over 50%). In addition, this DNA scaffold system enhanced the growth rate of the host strain by reducing the intracellular concentration of toxic intermediates, such as homoserine. Our DNA scaffold system can be used as a platform technology for the construction and optimization of artificial metabolic pathways as well as for the production of many useful biomaterials.
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المشرفين على المادة: 0 (Macromolecular Substances)
0 (Multienzyme Complexes)
2ZD004190S (Threonine)
9007-49-2 (DNA)
تواريخ الأحداث: Date Created: 20121120 Date Completed: 20130610 Latest Revision: 20220409
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC3568567
DOI: 10.1128/AEM.02578-12
PMID: 23160128
قاعدة البيانات: MEDLINE
الوصف
تدمد:1098-5336
DOI:10.1128/AEM.02578-12