دورية أكاديمية
أعرض في EDS

Ultra-bright and -stable red and near-infrared squaraine fluorophores for in vivo two-photon imaging.

التفاصيل البيبلوغرافية
العنوان: Ultra-bright and -stable red and near-infrared squaraine fluorophores for in vivo two-photon imaging.
المؤلفون: Podgorski K; Department of Cellular and Physiological Sciences and Brain Research Centre, University of British Columbia, Vancouver, British Columbia, Canada., Terpetschnig E, Klochko OP, Obukhova OM, Haas K
المصدر: PloS one [PLoS One] 2012; Vol. 7 (12), pp. e51980. Date of Electronic Publication: 2012 Dec 14.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Cyclobutanes/*chemistry , Fluorescent Dyes/*chemistry , Microscopy, Fluorescence, Multiphoton/*methods , Phenols/*chemistry, Animals ; Cyclobutanes/metabolism ; Fluorescent Dyes/metabolism ; Nerve Tissue Proteins/chemistry ; Nerve Tissue Proteins/metabolism ; Neurons/chemistry ; Neurons/metabolism ; Phenols/metabolism ; Photons ; Rotaxanes/chemistry ; Rotaxanes/metabolism ; SAP90-PSD95 Associated Proteins ; Synapses/chemistry ; Synapses/metabolism ; Xenopus laevis
مستخلص: Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons in vivo over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95.
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معلومات مُعتمدة: Canada Canadian Institutes of Health Research
المشرفين على المادة: 0 (Cyclobutanes)
0 (Fluorescent Dyes)
0 (Nerve Tissue Proteins)
0 (Phenols)
0 (Rotaxanes)
0 (SAP90-PSD95 Associated Proteins)
0 (squaraine)
تواريخ الأحداث: Date Created: 20121220 Date Completed: 20130905 Latest Revision: 20211021
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC3522634
DOI: 10.1371/journal.pone.0051980
PMID: 23251670
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0051980