دورية أكاديمية
Glycated collagen and altered glucose increase endothelial cell adhesion strength.
| العنوان: | Glycated collagen and altered glucose increase endothelial cell adhesion strength. |
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| المؤلفون: | Kemeny SF; Department of Mechanical Engineering and Mechanics, Drexel University, Philadelphia, Pennsylvania, USA., Cicalese S, Figueroa DS, Clyne AM |
| المصدر: | Journal of cellular physiology [J Cell Physiol] 2013 Aug; Vol. 228 (8), pp. 1727-36. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 0050222 Publication Model: Print Cited Medium: Internet ISSN: 1097-4652 (Electronic) Linking ISSN: 00219541 NLM ISO Abbreviation: J Cell Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Wiley-Liss Original Publication: Philadelphia, Wistar Institute of Anatomy and Biology. |
| مواضيع طبية MeSH: | Collagen/*metabolism , Endothelium, Vascular/*cytology , Endothelium, Vascular/*physiology , Glucose/*metabolism , Glycoproteins/*metabolism, Animals ; Biomechanical Phenomena ; Cell Adhesion/drug effects ; Cell Adhesion/physiology ; Cells, Cultured ; Coated Materials, Biocompatible ; Diabetic Angiopathies/etiology ; Diabetic Angiopathies/pathology ; Diabetic Angiopathies/physiopathology ; Endothelium, Vascular/pathology ; Glucose/chemistry ; Substrate Specificity/physiology ; Swine ; Vascular Endothelial Growth Factor A/metabolism |
| مستخلص: | Cell adhesion strength is important to cell survival, proliferation, migration, and mechanotransduction, yet changes in endothelial cell adhesion strength have not yet been examined in diseases such as diabetes with high rates of cardiovascular complications. We therefore investigated porcine aortic endothelial cell adhesion strength on native and glycated collagen-coated substrates and in low, normal, and high glucose culture using a spinning disc apparatus. Adhesion strength increased by 30 dynes/cm(2) in cells on glycated collagen as compared to native collagen. Attachment studies revealed that cells use higher adhesion strength αv β3 integrins to bind to glycated collagen instead of the typical α2 β1 integrins used to bind to native collagen. Similarly, endothelial cells cultured in low and high glucose had 15 dynes/cm(2) higher adhesion strength than cells in normal glucose after 2 days. Increased adhesion strength was due to elevated VEGF release and intracellular PKC in low and high glucose cells, respectively. Thus glucose increased endothelial cell adhesion strength via different underlying mechanisms. These adhesion strength changes could contribute to diabetic vascular disease, including accelerated atherosclerosis and disordered angiogenesis. (Copyright © 2013 Wiley Periodicals, Inc.) |
| المشرفين على المادة: | 0 (Coated Materials, Biocompatible) 0 (Glycoproteins) 0 (Vascular Endothelial Growth Factor A) 9007-34-5 (Collagen) IY9XDZ35W2 (Glucose) |
| تواريخ الأحداث: | Date Created: 20130103 Date Completed: 20130624 Latest Revision: 20181202 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1002/jcp.24313 |
| PMID: | 23280505 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1097-4652 |
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| DOI: | 10.1002/jcp.24313 |