دورية أكاديمية
Volume replacement with saline solutions during pancreatitis in rats and the hepatic profiles of apoptotic proteins and heat-shock proteins.
| العنوان: | Volume replacement with saline solutions during pancreatitis in rats and the hepatic profiles of apoptotic proteins and heat-shock proteins. |
|---|---|
| المؤلفون: | Rios EC; Laboratory of Medical Investigation (LIM-51), Department of Emergency Medicine, Faculdadede Medicina, Universidade de São Paulo - USP -São Paulo (SP), Brazil., Moretti AI, Souza HP, Velasco IT, Soriano FG |
| المصدر: | Revista Brasileira de terapia intensiva [Rev Bras Ter Intensiva] 2012 Dec; Vol. 24 (4), pp. 326-33. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Associação de Medicina Intensiva Brasileira Country of Publication: Brazil NLM ID: 9506692 Publication Model: Print Cited Medium: Internet ISSN: 1982-4335 (Electronic) Linking ISSN: 0103507X NLM ISO Abbreviation: Rev Bras Ter Intensiva Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: [Rio de Janeiro] : Associação de Medicina Intensiva Brasileira, |
| مستخلص: | Objective: Liver failure can occur as a consequence of the systemic inflammation after acute pancreatitis. We assessed the effect of volume repositioning with hypertonic saline solution or normal saline on hepatic cytokine production and the expression of heat-shock proteins and apoptotic proteins after acute pancreatitis. Methods: Wistar rats were divided in four groups: C - control animals that were not subjected to insult or treatment; NT - animals that were subjected to acute pancreatitis and received no treatment; normal saline - animals that were subjected to acute pancreatitis and received normal saline (NaCl 0.9%); and HS - animals that were subjected to acute pancreatitis and received hypertonic saline solution (NaCl 7.5%). Acute pancreatitis was induced by retrograde transduodenal infusion of 2.5% sodium taurocholate into the pancreatic duct. At 4, 12 and 24 h following acute pancreatitis induction, TNF-alpha, IL-1-beta, IL-6 and IL-10, caspase-2 and -7, Apaf-1, AIF and HSP60 and 90 were analyzed in the liver. Results: Casp2 decreased in the normal saline and hypertonic saline groups (p<0.05 versus. C) at 12 h. Apaf-1, AIF and HSP90 remained unchanged. At 4 h, Casp7 increased in the NT group (p<0.01 versus C), although it remained at the baseline levels in the reperfused groups. HSP60 increased in all of the groups at 4 h (p< 0.001 vs. C). However, the hypertonic saline group showed lower expression of HSP60 than the normal saline group (p<0.05). Hypertonic saline solution maintained the production of cytokines at normal levels. Volume reperfusion with normal or hypertonic saline significantly modulated the expression of Casp7. Conclusion: Volume replacement with hypertonic or normal saline was effective in reducing caspase 7. However, only hypertonic solution was capable of regulating cytokine production and HSP60 expression at all time points. |
| التعليقات: | Comment in: Rev Bras Ter Intensiva. 2012 Dec;24(4):315. (PMID: 23917923) |
| References: | Life Sci. 2007 Apr 24;80(20):1839-45. (PMID: 17367817) Hepatology. 2006 Feb;43(2 Suppl 1):S31-44. (PMID: 16447272) Resuscitation. 2002 Dec;55(3):329-36. (PMID: 12458070) J Hepatol. 2002 Sep;37(3):400-10. (PMID: 12175638) Exp Cell Res. 2000 Apr 10;256(1):67-73. (PMID: 10739653) Biochem Biophys Res Commun. 2009 Sep 25;387(3):553-7. (PMID: 19615333) Am J Physiol. 1997 Oct;273(4):G937-45. (PMID: 9357838) Biochem Biophys Res Commun. 1997 Nov 17;240(2):419-24. (PMID: 9388494) Clinics (Sao Paulo). 2011;66(3):469-76. (PMID: 21552675) Clin Exp Pharmacol Physiol. 2010 Jan;37(1):35-9. (PMID: 19515067) Am J Physiol Gastrointest Liver Physiol. 2004 Feb;286(2):G189-96. (PMID: 14715516) Endocrinology. 2001 Jun;142(6):2468-80. (PMID: 11356696) Cell Death Differ. 1999 Oct;6(10):937-42. (PMID: 10556969) Eur J Pharmacol. 2008 Dec 28;601(1-3):136-42. (PMID: 18977215) Am J Physiol. 1980 Nov;239(5):H664-73. (PMID: 6776826) J Gastrointest Surg. 2002 May-Jun;6(3):474-80. (PMID: 12023002) Shock. 2002 Feb;17(2):91-7. (PMID: 11837795) J Gastroenterol. 2005 Jan;40(1):1-10. (PMID: 15692783) Am J Physiol Cell Physiol. 2004 Apr;286(4):C739-44. (PMID: 15001423) Rev Bras Ter Intensiva. 2008 Sep;20(3):267-77. (PMID: 25307095) Surgery. 2000 Jan;127(1):55-64. (PMID: 10660759) Surgery. 2000 Jun;127(6):641-9. (PMID: 10840359) Cell Death Differ. 2007 Jul;14(7):1285-94. (PMID: 17431416) Shock. 2012 Mar;37(3):268-75. (PMID: 22089205) Pancreas. 2009 Jul;38(5):507-14. (PMID: 19346995) Pancreas. 2006 Jan;32(1):80-6. (PMID: 16340748) Oncogene. 2003 Dec 8;22(56):9041-7. (PMID: 14663482) Cancer Res. 2003 Aug 1;63(15):4368-74. (PMID: 12907606) J Exp Med. 2005 Apr 4;201(7):1135-43. (PMID: 15795240) Nat Rev Immunol. 2006 Nov;6(11):813-22. (PMID: 17039247) |
| تواريخ الأحداث: | Date Created: 20130807 Date Completed: 20131018 Latest Revision: 20211021 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC4031807 |
| DOI: | 10.1590/s0103-507x2012000400006 |
| PMID: | 23917928 |
| قاعدة البيانات: | MEDLINE |
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