دورية أكاديمية
Molecular characterization of the circulating strains of Vibrio cholerae during 2010 cholera outbreak in Nigeria.
| العنوان: | Molecular characterization of the circulating strains of Vibrio cholerae during 2010 cholera outbreak in Nigeria. |
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| المؤلفون: | Oyedeji KS; Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, Yaba, Lagos., Niemogha MT, Nwaokorie FO, Bamidele TA, Ochoga M, Akinsinde KA, Brai BI, Oladele D, Omonigbehin EA, Bamidele M, Fesobi TW, Musa AZ, Adeneye AK, Smith SI, Ujah IA |
| المصدر: | Journal of health, population, and nutrition [J Health Popul Nutr] 2013 Jun; Vol. 31 (2), pp. 178-84. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMedCentral Country of Publication: Bangladesh NLM ID: 100959228 Publication Model: Print Cited Medium: Print ISSN: 1606-0997 (Print) Linking ISSN: 16060997 NLM ISO Abbreviation: J Health Popul Nutr Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: May 2015- : London: BioMedCentral Original Publication: Dhaka, Bangladesh : ICDDR,B: Centre for Health and Population Research, c2000- |
| مواضيع طبية MeSH: | Cholera/*epidemiology , Disease Outbreaks/*statistics & numerical data , Molecular Typing/*methods , Vibrio cholerae/*isolation & purification, Cholera/blood ; DNA, Bacterial/genetics ; DNA, Bacterial/isolation & purification ; Humans ; Nigeria/epidemiology ; Polymerase Chain Reaction/methods ; Vibrio cholerae/classification ; Vibrio cholerae/genetics |
| مستخلص: | This study aimed at characterizing the phenotypic and toxigenic status of circulating strains of cholera during outbreaks in Nigeria, employing molecular typing techniques. Two hundred and one samples of rectal swabs, stool, vomitus, water (from the well, borehole, sachet, stream, and tap) and disinfectants (sodium hypochlorite) were collected from three states in the country. The samples were inoculated on thiosulphate-citrate bile salt-sucrose (TCBS), Cary-Blair transport medium and smeared on glass slides for direct examination. The Vibrio cholerae isolates were serotyped, biotyped, and characterized using PCR of the cytotoxin gene A (ctxA), wbeO1, and wbfO139 gene primer. Of the 201 samples screened, 96 were positive for V cholerae O1 (48%), with 69 (72%) positive for ctxA gene. The results from this study showed that the circulating strains of cholera in Nigeria were of Ogawa serotype, also observed in other outbreaks in Nigeria (1991, 1992, and 1996). However, the strains were of the Classical biotype and were mainly (72%) ctxA gene-positive. This current investigation has confirmed the production of cholera toxin by the circulating strains, and this could be harnessed for possible cholera vaccine production in Nigeria. |
| References: | Appl Environ Microbiol. 2004 Apr;70(4):1964-72. (PMID: 15066786) Microbiol Mol Biol Rev. 1998 Dec;62(4):1301-14. (PMID: 9841673) J Hyg (Lond). 1983 Apr;90(2):199-205. (PMID: 6833745) Wkly Epidemiol Rec. 1998 Jul 3;73(27):201-8. (PMID: 9677852) J Clin Microbiol. 2010 Nov;48(11):3918-22. (PMID: 20739485) Emerg Infect Dis. 2011 Nov;17(11):2026-34. (PMID: 22099090) Emerg Infect Dis. 2011 Nov;17(11):2151-4. (PMID: 22099122) J Health Popul Nutr. 2008 Mar;26(1):79-87. (PMID: 18637531) Wkly Epidemiol Rec. 2001 Aug 3;76(31):233-40. (PMID: 11505731) Emerg Infect Dis. 2011 Mar;17(3):568-9. (PMID: 21392467) Emerg Infect Dis. 2011 Nov;17(11):2122-9. (PMID: 22099116) J Clin Microbiol. 2010 Oct;48(10):3666-74. (PMID: 20668130) J Clin Microbiol. 2010 Nov;48(11):3963-9. (PMID: 20826646) J Clin Microbiol. 2000 Oct;38(10):3774-9. (PMID: 11015401) Wkly Epidemiol Rec. 2010 Mar 26;85(13):117-28. (PMID: 20349546) Am J Trop Med Hyg. 2006 Nov;75(5):973-7. (PMID: 17123999) FEMS Immunol Med Microbiol. 1998 Mar;20(3):201-7. (PMID: 9566491) Clin Microbiol Rev. 1995 Jan;8(1):48-86. (PMID: 7704895) J Infect Dis. 1994 May;169(5):1029-34. (PMID: 8169387) |
| المشرفين على المادة: | 0 (DNA, Bacterial) |
| تواريخ الأحداث: | Date Created: 20130813 Date Completed: 20130822 Latest Revision: 20211021 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC3702338 |
| DOI: | 10.3329/jhpn.v31i2.16381 |
| PMID: | 23930335 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1606-0997 |
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| DOI: | 10.3329/jhpn.v31i2.16381 |