دورية أكاديمية
Enhanced diagnostic yields of bacteremia and candidemia in blood specimens by PCR-electrospray ionization mass spectrometry.
| العنوان: | Enhanced diagnostic yields of bacteremia and candidemia in blood specimens by PCR-electrospray ionization mass spectrometry. |
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| المؤلفون: | Laffler TG; Ibis Biosciences, Carlsbad, California, USA., Cummins LL, McClain CM, Quinn CD, Toro MA, Carolan HE, Toleno DM, Rounds MA, Eshoo MW, Stratton CW, Sampath R, Blyn LB, Ecker DJ, Tang YW |
| المصدر: | Journal of clinical microbiology [J Clin Microbiol] 2013 Nov; Vol. 51 (11), pp. 3535-41. Date of Electronic Publication: 2013 Aug 21. |
| نوع المنشور: | Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7505564 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-660X (Electronic) Linking ISSN: 00951137 NLM ISO Abbreviation: J Clin Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Bacteremia/*diagnosis , Candidemia/*diagnosis , Microbiological Techniques/*methods , Molecular Diagnostic Techniques/*methods , Polymerase Chain Reaction/*methods , Spectrometry, Mass, Electrospray Ionization/*methods, Adult ; Aged ; Bacteria/classification ; Bacteria/isolation & purification ; Blood/microbiology ; Candida/classification ; Candida/isolation & purification ; Female ; Humans ; Male ; Middle Aged ; Prospective Studies ; Time Factors |
| مستخلص: | A prospective study was performed to determine the value of direct molecular testing of whole blood for detecting the presence of culturable and unculturable bacteria and yeasts in patients with suspected bloodstream infections. A total of 464 adult and pediatric patients with positive blood cultures matched with 442 patients with negative blood cultures collected during the same period were recruited during a 10-month study. PCR amplification coupled with electrospray ionization mass spectrometry (PCR-ESI-MS) plus blood culture reached an overall agreement of 78.6% in the detection and species-level identification of bacterial and candidal pathogens. Of 33 culture-negative/PCR-ESI-MS-positive specimens, 31 (93.9%) were judged to be truly bacteremic and/or candidemic based on a medical chart review and analytical metrics. Among the 15 culture-positive specimens in which PCR-ESI-MS detected additional bacterial or yeast species, 66.7% and 20.0% of the additional positive specimens by PCR-ESI-MS were judged to be truly or possibly bacteremic and/or candidemic, respectively. Direct analysis of blood samples by PCR-ESI-MS rapidly detects bacterial and yeast pathogens in patients with bloodstream infections. When used in conjunction with blood culture, PCR-ESI-MS enhances the diagnostics of septicemia by shortening test turnaround time and improving yields. |
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| تواريخ الأحداث: | Date Created: 20130823 Date Completed: 20140331 Latest Revision: 20240519 |
| رمز التحديث: | 20240519 |
| مُعرف محوري في PubMed: | PMC3889730 |
| DOI: | 10.1128/JCM.00876-13 |
| PMID: | 23966503 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-660X |
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| DOI: | 10.1128/JCM.00876-13 |