دورية أكاديمية
A novel non-toxic xylene substitute (SBO) for histology.
| العنوان: | A novel non-toxic xylene substitute (SBO) for histology. |
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| المؤلفون: | Kunhua W; The First People's Hospital of Yunnan Province, Kunming 650032, PR China., Chuming F, Tao L, Yanmei Y, Xin Y, Xiaoming Z, Xuezhong G, Xun L |
| المصدر: | African journal of traditional, complementary, and alternative medicines : AJTCAM [Afr J Tradit Complement Altern Med] 2011 Oct 02; Vol. 9 (1), pp. 43-9. Date of Electronic Publication: 2011 Oct 02 (Print Publication: 2012). |
| نوع المنشور: | Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: African Traditional Herbal Medicine Supporters Initiative Country of Publication: Nigeria NLM ID: 101232990 Publication Model: eCollection Cited Medium: Internet ISSN: 2505-0044 (Electronic) Linking ISSN: 25050044 NLM ISO Abbreviation: Afr J Tradit Complement Altern Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Osun : African Traditional Herbal Medicine Supporters Initiative Original Publication: [Ile-Ife, Nigeria? : African Networks on Ethnomedicines, 2004]- |
| مواضيع طبية MeSH: | Histology* , Pathology* , Xylenes*, Laboratory Chemicals/*chemistry, Animals ; Humans ; Rats |
| مستخلص: | Xylene has been generally used as a clearing and deparaffinizing agent in histology. Because of the potential toxic and flammable nature of xylene, its substitutes have been introduced into some laboratories. In this study, we introduced a novel, non-toxic xylene substitute (SBO), which was generated through a mixture of 86% of white oil No.2 and 14% of N-heptane. SBO had a high boiling point (188°C) and flash point (144°C) coupled with a scentless and decreased volatility. To compare the effectiveness of SBO and xylene in histology, a wide range of tissue samples from rats and human beings were processed in parallel in SBO and xylene, subjected to various staining procedures. Similar to the xylene-processed paraffin blocks, the SBO-processed counterparts were easy to section without any evidence of cell shrinkage. Assessment of the SBO-treated sections stained with hematoxylin-eosin revealed a good maintenance of cell morphology and structure, and a clear definition of the cytoplasm and the nucleus. Moreover, comparable good results were achieved between the SBO- and xylene-processed tissues in other histochemical and immunohistochemical stainings. Six-month clinical applications at one department of pathology supported the potentials of SBO as a xylene substitute. In conclusion, we suggest that SBO is a safe and efficient substitute of xylene and may probably replace xylene without losing valuable diagnostic information. |
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| فهرسة مساهمة: | Keywords: SBO; clearing agent; histology; toxicity; xylene |
| المشرفين على المادة: | 0 (Laboratory Chemicals) 0 (Xylenes) |
| تواريخ الأحداث: | Date Created: 20130829 Date Completed: 20140407 Latest Revision: 20220408 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC3746537 |
| DOI: | 10.4314/ajtcam.v9i1.6 |
| PMID: | 23983318 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2505-0044 |
|---|---|
| DOI: | 10.4314/ajtcam.v9i1.6 |