دورية أكاديمية
Lentivirus-based RNA silencing of Nemo-like kinase (NLK) inhibits the CAL 27 human adenosquamos carcinoma cells proliferation and blocks G0/G1 phase to S phase.
| العنوان: | Lentivirus-based RNA silencing of Nemo-like kinase (NLK) inhibits the CAL 27 human adenosquamos carcinoma cells proliferation and blocks G0/G1 phase to S phase. |
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| المؤلفون: | Zhang B; Department of Anatomy Shandong University, School of Medicine, Jinan Shandong, 250012, P R China., Li KY, Chen HY, Pan SD, Chen SF, Zhang WF, Xia CP, Jiang LC, Liu XB, Zhao FJ, Yuan DY, Wang le X, Wu YP, Liu SW |
| المصدر: | International journal of medical sciences [Int J Med Sci] 2013 Aug 08; Vol. 10 (10), pp. 1301-6. Date of Electronic Publication: 2013 Aug 08 (Print Publication: 2013). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Ivyspring International Publisher Country of Publication: Australia NLM ID: 101213954 Publication Model: eCollection Cited Medium: Internet ISSN: 1449-1907 (Electronic) Linking ISSN: 14491907 NLM ISO Abbreviation: Int J Med Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Australia] : Ivyspring International Publisher, c2004- |
| مواضيع طبية MeSH: | Carcinoma, Adenosquamous/*enzymology , G1 Phase/*physiology , Intracellular Signaling Peptides and Proteins/*metabolism , Lentivirus/*genetics , Protein Serine-Threonine Kinases/*metabolism , RNA Interference/*physiology , Resting Phase, Cell Cycle/*physiology , S Phase/*physiology, Cell Line, Tumor ; Cell Proliferation ; G1 Phase/genetics ; Humans ; Intracellular Signaling Peptides and Proteins/genetics ; Protein Serine-Threonine Kinases/genetics ; Resting Phase, Cell Cycle/genetics ; S Phase/genetics |
| مستخلص: | Background: The Nemo-like kinase (NLK) is a serine/threonine-protein kinase that involved in a number of signaling pathways regulating cell fate. Variation of NLK has been shown to be associated with the risk of cancer. However, the function of NLK in oral adenosquamous carcinoma cells line CAL-27 is unknown. Methods: In this study, we evaluated the function of NLK in CAL-27 cells by using lentivirus-mediated RNA silence. The targeted gene expression, cell proliferation and cell cycle are investigated by RT-PCR, western-blot, MTT method, colony forming assay and flow cytometry analysis respectively. Results: After NLK silencing, the number of colonies was significantly reduced (54 ± 5 colonies/well compared with 262 ± 18 colonies/well in non-infected or 226 ± 4 colonies/well in negative control group (sequence not related to NLK sequence with mismatched bases). Using crystal violet staining, we also found that the cell number per colony was dramatically reduced. The RNA silencing of NLK blocks the G0/G1 phase to S phase progression during the cell cycle. Conclusions: These results suggest that NLK silencing by lentivirus-mediated RNA interference would be a potential therapeutic method to control oral squamous carcinoma growth. |
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| فهرسة مساهمة: | Keywords: Lentivirus; Nemo-like kinase (NLK); Oral Squamous Cell Carcinoma; RNAi |
| المشرفين على المادة: | 0 (Intracellular Signaling Peptides and Proteins) EC 2.7.1.- (NLK protein, human) EC 2.7.11.1 (Protein Serine-Threonine Kinases) |
| تواريخ الأحداث: | Date Created: 20130829 Date Completed: 20140317 Latest Revision: 20211203 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC3753414 |
| DOI: | 10.7150/ijms.6607 |
| PMID: | 23983589 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1449-1907 |
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| DOI: | 10.7150/ijms.6607 |