دورية أكاديمية
أعرض في EDS

Genotypic diversity of Pseudomonas aeruginosa in cystic fibrosis siblings in Qatar using AFLP fingerprinting.

التفاصيل البيبلوغرافية
العنوان: Genotypic diversity of Pseudomonas aeruginosa in cystic fibrosis siblings in Qatar using AFLP fingerprinting.
المؤلفون: Abdul Wahab A; Hamad Medical Corporation, Department of Pediatrics, Hamad Medical Corporation, Doha, Qatar, atiqaaw@yahoo.com., Taj-Aldeen SJ, Hagen F, Diophode S, Saadoon A, Meis JF, Klaassen CH
المصدر: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2014 Feb; Vol. 33 (2), pp. 265-71. Date of Electronic Publication: 2013 Sep 01.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: Germany NLM ID: 8804297 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1435-4373 (Electronic) Linking ISSN: 09349723 NLM ISO Abbreviation: Eur J Clin Microbiol Infect Dis Subsets: MEDLINE
أسماء مطبوعة: Publication: Berlin : Springer
Original Publication: [Wiesbaden, Federal Republic of Germany] : Vieweg, [c1988-
مواضيع طبية MeSH: Amplified Fragment Length Polymorphism Analysis* , Genetic Variation* , Molecular Typing*, Cystic Fibrosis/*complications , Pseudomonas Infections/*microbiology , Pseudomonas aeruginosa/*classification , Pseudomonas aeruginosa/*genetics, Adolescent ; Adult ; Child ; Child, Preschool ; Female ; Genotype ; Humans ; Male ; Molecular Epidemiology ; Prospective Studies ; Pseudomonas Infections/transmission ; Pseudomonas aeruginosa/isolation & purification ; Qatar ; Siblings ; Young Adult
مستخلص: Pseudomonas aeruginosa is one of the primary pathogens in patients with cystic fibrosis (CF) and a major cause of morbidity and mortality. Reports of the spread of epidemic or transmissible strains of P. aeruginosa within and across CF centers raised the possibility of clonal spread among siblings with CF. This work reports the genotypic relatedness of P. aeruginosa in CF patients with the CFTR I1234V mutation, and to determine whether the genotypes are identical among CF siblings and among different families with the same CFTR mutation. Sixty-six P. aeruginosa isolates were obtained from sputa/deep-pharyngeal swabs from 27 CF patients belonging to 17 families. Genotypic relatedness was assessed using amplified fragment-length polymorphism (AFLP) fingerprinting. Twenty-three distinct genotypes of P. aeruginosa were identified. Eleven families each had one distinct genotype. In the other 6 families more than one genotype was observed; 3 families each showed two genotypes, 2 families each had three genotypes and 1 family had four genotypes of P. aeruginosa. In several cases, siblings with CF from the same family harbored the same strain of P. aeruginosa, which were different from the genotypes in other families. On the other hand, there was an overlap in P. aeruginosa between closely related families. Some patients show persistent colonization with the same genotype of P. aeruginosa over the longitudinal period. The presence of the same genotypes in siblings of the same family and closely related families suggests cross-transmission of P. aeruginosa or acquisition from common environmental exposure.
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تواريخ الأحداث: Date Created: 20130903 Date Completed: 20140915 Latest Revision: 20211021
رمز التحديث: 20231215
DOI: 10.1007/s10096-013-1954-1
PMID: 23996049
قاعدة البيانات: MEDLINE
الوصف
تدمد:1435-4373
DOI:10.1007/s10096-013-1954-1