دورية أكاديمية
Systemic treatment with 4-211Atphenylalanine enhances survival of rats with intracranial glioblastoma.
| العنوان: | Systemic treatment with 4-211Atphenylalanine enhances survival of rats with intracranial glioblastoma. |
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| المؤلفون: | Borrmann N, Friedrich S, Schwabe K, Hedrich HJ, Krauss JK, Knapp WH, Nakamura M, Meyer GJ, Walte A; Dr. Almut Walte, Klinik für Nuklearmedizin, Medizinische Hochschule Hannover, Carl-Neuberg-Str. 1 30625 Hannover, Germany, Tel. +49/(0)511/532 40 11, E-mail: walte.almut@mh-hannover.de. |
| المصدر: | Nuklearmedizin. Nuclear medicine [Nuklearmedizin] 2013 Dec 13; Vol. 52 (6), pp. 212-21. Date of Electronic Publication: 2013 Sep 06. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Thieme Country of Publication: Germany NLM ID: 7609387 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2567-6407 (Electronic) Linking ISSN: 00295566 NLM ISO Abbreviation: Nuklearmedizin Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2018- : Stuttgart : Thieme Original Publication: Stuttgart, F.K. Schattauer. |
| مواضيع طبية MeSH: | Astatine/*administration & dosage , Brachytherapy/*methods , Brain Neoplasms/*drug therapy , Glioblastoma/*drug therapy , Phenylalanine/*administration & dosage, Animals ; Brain Neoplasms/diagnosis ; Cell Line, Tumor ; Dose-Response Relationship, Radiation ; Female ; Glioblastoma/diagnosis ; Radiopharmaceuticals/administration & dosage ; Rats ; Survival Rate ; Treatment Outcome |
| مستخلص: | Objective: Increased amino acid transport in brain tumours is used for diagnostic purposes. It has been shown that the α-emitting radionuclide astatine-211 labeled to L-phenylalanine is taken up by glioblastoma cells. We here tested, if systemic treatment with 4-[211At]astatine-phenylalanine (At-Phe) has a beneficial effect on survival of rats with intracranial glioblastoma. Animals, Methods: The rat glioblastoma cell line BT4Ca was implanted into the prefrontal cortex of female BDIX rats by stereotaxic microinjection (10,000 cells/3 µl; n = 83). 3 days after implantation At-Phe or phosphate buffered saline were injected intravenously. A third group was treated twice, i.e., on day 3 and 10. Health condition was assessed each day by using a score system. Rats were sacrificed on days 6, 10, 13 and 17 after implantation, or when showing premortal health condition to measure tumour volume and necrosis. The proliferation index (PI) was assessed after immunohistochemical staining of Ki-67. Results: Survival time of rats treated twice with At-Phe was significantly prolonged. Additionally, both At-Phe-treated groups remained significantly longer in a better health condition. Rats with poor health status had larger tumours than rats with fair health condition. Overall, irrespective of treatment the PI was reduced in rats with poor health condition. Necrosis was larger in rats treated twice with At-Phe. Conclusion: Intravenous treatment with At-Phe enhanced survival time of rats with intracranial glioblastomas and improved health condition. These results encourage studies using local treatment of intracranial glioblastoma with At-Phe, either by repeated local injection or by intracavital application after tumour resection. |
| فهرسة مساهمة: | Keywords: At-phenylalanine; Glioblastoma; astatine-211; intracranial glioblastoma rat model |
| المشرفين على المادة: | 0 (Radiopharmaceuticals) 47E5O17Y3R (Phenylalanine) XI595HAL7H (Astatine) |
| تواريخ الأحداث: | Date Created: 20130917 Date Completed: 20140806 Latest Revision: 20141113 |
| رمز التحديث: | 20240628 |
| DOI: | 10.3413/Nukmed-0580-13-05 |
| PMID: | 24036694 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2567-6407 |
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| DOI: | 10.3413/Nukmed-0580-13-05 |