دورية أكاديمية
أعرض في EDS

Sources and structures of mitotic crossovers that arise when BLM helicase is absent in Drosophila.

التفاصيل البيبلوغرافية
العنوان: Sources and structures of mitotic crossovers that arise when BLM helicase is absent in Drosophila.
المؤلفون: LaFave MC; Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599., Andersen SL, Stoffregen EP, Holsclaw JK, Kohl KP, Overton LJ, Sekelsky J
المصدر: Genetics [Genetics] 2014 Jan; Vol. 196 (1), pp. 107-18. Date of Electronic Publication: 2013 Oct 30.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: United States NLM ID: 0374636 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1943-2631 (Electronic) Linking ISSN: 00166731 NLM ISO Abbreviation: Genetics Subsets: MEDLINE
أسماء مطبوعة: Publication: 2021- : [Oxford] : Oxford University Press
Original Publication: Austin, Tex. [etc.]
مواضيع طبية MeSH: Crossing Over, Genetic/*genetics , DNA End-Joining Repair/*genetics , DNA Helicases/*genetics , Drosophila Proteins/*genetics , Drosophila melanogaster/*genetics, Animals ; Base Sequence ; Crossing Over, Genetic/drug effects ; DNA Breaks, Double-Stranded ; DNA Repair/genetics ; Female ; Hydroxyurea/pharmacology ; Male ; Mitosis/genetics ; Nucleic Acid Synthesis Inhibitors/pharmacology ; Sequence Analysis, DNA
مستخلص: The Bloom syndrome helicase, BLM, has numerous functions that prevent mitotic crossovers. We used unique features of Drosophila melanogaster to investigate origins and properties of mitotic crossovers that occur when BLM is absent. Induction of lesions that block replication forks increased crossover frequencies, consistent with functions for BLM in responding to fork blockage. In contrast, treatment with hydroxyurea, which stalls forks, did not elevate crossovers, even though mutants lacking BLM are sensitive to killing by this agent. To learn about sources of spontaneous recombination, we mapped mitotic crossovers in mutants lacking BLM. In the male germline, irradiation-induced crossovers were distributed randomly across the euchromatin, but spontaneous crossovers were nonrandom. We suggest that regions of the genome with a high frequency of mitotic crossovers may be analogous to common fragile sites in the human genome. Interestingly, in the male germline there is a paucity of crossovers in the interval that spans the pericentric heterochromatin, but in the female germline this interval is more prone to crossing over. Finally, our system allowed us to recover pairs of reciprocal crossover chromosomes. Sequencing of these revealed the existence of gene conversion tracts and did not provide any evidence for mutations associated with crossovers. These findings provide important new insights into sources and structures of mitotic crossovers and functions of BLM helicase.
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معلومات مُعتمدة: P30 ES010126 United States ES NIEHS NIH HHS; R01 GM061252. United States GM NIGMS NIH HHS; K12 GM000678 United States GM NIGMS NIH HHS; T32 GM007092 United States GM NIGMS NIH HHS; R01 GM061252 United States GM NIGMS NIH HHS; R01 GM099890 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: BLM helicase; crossovers; mitotic recombination
المشرفين على المادة: 0 (Drosophila Proteins)
0 (Nucleic Acid Synthesis Inhibitors)
EC 3.6.4.- (DNA Helicases)
EC 3.6.4.12 (blm protein, Drosophila)
X6Q56QN5QC (Hydroxyurea)
تواريخ الأحداث: Date Created: 20131101 Date Completed: 20140902 Latest Revision: 20211021
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC3872178
DOI: 10.1534/genetics.113.158618
PMID: 24172129
قاعدة البيانات: MEDLINE
الوصف
تدمد:1943-2631
DOI:10.1534/genetics.113.158618