التفاصيل البيبلوغرافية
| العنوان: |
Detection of K-ras oncogene from the human genomic DNA using ultrasonication and a quantum dots-based microfluidic chip. |
| المؤلفون: |
Noh HN; Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-do 461-701, Republic of Korea., Kim JS |
| المصدر: |
Journal of nanoscience and nanotechnology [J Nanosci Nanotechnol] 2013 Sep; Vol. 13 (9), pp. 6033-7. |
| نوع المنشور: |
Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: American Scientific Publishers Country of Publication: United States NLM ID: 101088195 Publication Model: Print Cited Medium: Print ISSN: 1533-4880 (Print) Linking ISSN: 15334880 NLM ISO Abbreviation: J Nanosci Nanotechnol Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Stevenson Ranch, CA : American Scientific Publishers, 2001-2021 |
| مواضيع طبية MeSH: |
Genes, ras* , Genome, Human* , Quantum Dots* , Ultrasonics*, DNA/*genetics , Microfluidics/*instrumentation, Humans ; Microscopy, Fluorescence ; Mutation |
| مستخلص: |
In clinical diagnostics, single-stranded DNAs (ssDNA) have been prepared from the human genomic DNA for the detection of a specific gene. In this study, the human genomic DNA was degraded via ultrasonication in solution, and K-ras oncogene was detected from the DNA fragments via the fluorescence quenching of quantum dots (QDs) by intercalating dyes after hybridization of the target, to probe DNAs in a microfluidic chip. K-ras is one of the most activated common oncogenes, and many human tumors are known to be due to the mutation of this gene. QDs are nano-sized semiconductors with a wide selection of emission wavelengths and exceptional stability against photo bleaching. In this study, probe DNA-conjugated QDs were immobilized to polystyrene microbeads, and the DNA-microbead-QDs complexes were packed through a microchannel by pillars that trap the beads in the microfluidic chip. The fluorescence of the QDs could be quenched by intercalating dye (TOTO-3) after hybridization of K-ras oncogene to the probe DNA in the channel. The fluorescence intensity decrease of the QDs can be used as an indication of the K-ras oncogene. By introducing an alkaline buffer solution, the DNAs were denatured, and the fluorescence intensity of the QDs again increased, which shows the possibility of reuse of the microfluidic chip for the detection of the K-ras gene. |
| المشرفين على المادة: |
9007-49-2 (DNA) |
| تواريخ الأحداث: |
Date Created: 20131112 Date Completed: 20140108 Latest Revision: 20190715 |
| رمز التحديث: |
20231215 |
| DOI: |
10.1166/jnn.2013.7654 |
| PMID: |
24205594 |
| قاعدة البيانات: |
MEDLINE |
