دورية أكاديمية
أعرض في EDS

Newly-derived neuroblastoma cell lines propagated in serum-free media recapitulate the genotype and phenotype of primary neuroblastoma tumours.

التفاصيل البيبلوغرافية
العنوان: Newly-derived neuroblastoma cell lines propagated in serum-free media recapitulate the genotype and phenotype of primary neuroblastoma tumours.
المؤلفون: Bate-Eya LT; Department of Oncogenomics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, PO Box 22700, 1105 AZ Amsterdam, The Netherlands., Ebus ME; Department of Oncogenomics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, PO Box 22700, 1105 AZ Amsterdam, The Netherlands., Koster J; Department of Oncogenomics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, PO Box 22700, 1105 AZ Amsterdam, The Netherlands., den Hartog IJ; Department of Oncogenomics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, PO Box 22700, 1105 AZ Amsterdam, The Netherlands., Zwijnenburg DA; Department of Oncogenomics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, PO Box 22700, 1105 AZ Amsterdam, The Netherlands., Schild L; Department of Oncogenomics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, PO Box 22700, 1105 AZ Amsterdam, The Netherlands., van der Ploeg I; Department of Oncogenomics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, PO Box 22700, 1105 AZ Amsterdam, The Netherlands., Dolman ME; Department of Oncogenomics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, PO Box 22700, 1105 AZ Amsterdam, The Netherlands., Caron HN; Department of Paediatric Oncology, Academic Medical Center, University of Amsterdam, Meibergdreef 15, PO Box 22700, 1105 AZ Amsterdam, The Netherlands., Versteeg R; Department of Oncogenomics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, PO Box 22700, 1105 AZ Amsterdam, The Netherlands., Molenaar JJ; Department of Oncogenomics, Academic Medical Center, University of Amsterdam, Meibergdreef 15, PO Box 22700, 1105 AZ Amsterdam, The Netherlands. Electronic address: j.j.molenaar@amc.uva.nl.
المصدر: European journal of cancer (Oxford, England : 1990) [Eur J Cancer] 2014 Feb; Vol. 50 (3), pp. 628-37. Date of Electronic Publication: 2013 Dec 06.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 9005373 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0852 (Electronic) Linking ISSN: 09598049 NLM ISO Abbreviation: Eur J Cancer Subsets: MEDLINE
أسماء مطبوعة: Publication: Oxford : Elsevier Science Ltd
Original Publication: Oxford ; New York : Pergamon Press, c1990-
مواضيع طبية MeSH: Cell Line, Tumor*, Neuroblastoma/*genetics , Neuroblastoma/*pathology, Animals ; Biomarkers, Tumor/genetics ; Biomarkers, Tumor/metabolism ; Child ; Child, Preschool ; Culture Media, Serum-Free ; Genotype ; Humans ; Infant ; Mice ; Mice, Nude ; Neuroblastoma/metabolism ; Phenotype ; RNA, Messenger/genetics ; RNA, Messenger/metabolism
مستخلص: Recently protocols have been devised for the culturing of cell lines from fresh tumours under serum-free conditions in defined neural stem cell medium. These cells, frequently called tumour initiating cells (TICs) closely retained characteristics of the tumours of origin. We report the isolation of eight newly-derived neuroblastoma TICs from six primary neuroblastoma tumours and two bone marrow metastases. The primary tumours from which these TICs were generated have previously been fully typed by whole genome sequencing (WGS). Array comparative genomic hybridisation (aCGH) analysis showed that TIC lines retained essential characteristics of the primary tumours and exhibited typical neuroblastoma chromosomal aberrations such as MYCN amplification, gain of chromosome 17q and deletion of 1p36. Protein analysis showed expression for neuroblastoma markers MYCN, NCAM, CHGA, DBH and TH while haematopoietic markers CD19 and CD11b were absent. We analysed the growth characteristics and confirmed tumour-forming potential using sphere-forming assays, subcutaneous and orthotopic injection of these cells into immune-compromised mice. Affymetrix mRNA expression profiling of TIC line xenografts showed an expression pattern more closely mimicking primary tumours compared to xenografts from classical cell lines. This establishes that these neuroblastoma TICs cultured under serum-free conditions are relevant and useful neuroblastoma tumour models.
(Copyright © 2013 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Neuroblastoma; Neuroblastoma TICs; Principal component analysis; Whole genome sequencing; aCGH
المشرفين على المادة: 0 (Biomarkers, Tumor)
0 (Culture Media, Serum-Free)
0 (RNA, Messenger)
تواريخ الأحداث: Date Created: 20131211 Date Completed: 20140609 Latest Revision: 20151119
رمز التحديث: 20231215
DOI: 10.1016/j.ejca.2013.11.015
PMID: 24321263
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0852
DOI:10.1016/j.ejca.2013.11.015