دورية أكاديمية
Detecting adenosine triphosphatase 6 (pfATP6) point mutations that may be associated with Plasmodium falciparum resistance to artemisinin: prevalence at baseline, before policy change in Uganda.
| العنوان: | Detecting adenosine triphosphatase 6 (pfATP6) point mutations that may be associated with Plasmodium falciparum resistance to artemisinin: prevalence at baseline, before policy change in Uganda. |
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| المؤلفون: | Kamugisha E; Department of Biochemistry, Bugando University College of Health Sciences, Mwanza, Tanzania. erasmuskamugisha@yahoo.com, Sendagire H; Department of Microbiology, Makerere University-Kampala, Uganda., Kaddumukasa M; Department of Medicine, Makerere University, Kampala, Uganda., Enweji N; Department of Medical Biochemistry and Microbiology, Uppsala University, Sweden., Gheysari F; Department of Medical Biochemistry and Microbiology, Uppsala University, Sweden., Swedberg G; Department of Medical Biochemistry and Microbiology, Uppsala University, Sweden., Kironde F; Department of Microbiology, Makerere University-Kampala, Uganda. |
| المصدر: | Tanzania journal of health research [Tanzan J Health Res] 2011 Jan; Vol. 13 (1), pp. 40-7. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Health Research User's Trust Fund Country of Publication: Tanzania NLM ID: 101479163 Publication Model: Print Cited Medium: Print ISSN: 1821-6404 (Print) Linking ISSN: 18219241 NLM ISO Abbreviation: Tanzan J Health Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Dar es Salaam : Health Research User's Trust Fund |
| مواضيع طبية MeSH: | Anti-Infective Agents/*pharmacology , Artemisinins/*pharmacology , Calcium-Transporting ATPases/*genetics , Drug Resistance, Microbial/*genetics , Malaria, Falciparum/*drug therapy, Anti-Infective Agents/administration & dosage ; Artemether ; Artemisinins/administration & dosage ; Drug Therapy, Combination ; Ethanolamines/administration & dosage ; Fluorenes/administration & dosage ; Genotype ; Humans ; Lumefantrine ; Malaria, Falciparum/epidemiology ; Point Mutation ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Prevalence ; Sequence Analysis, DNA ; Uganda/epidemiology |
| مستخلص: | The artemisinin based combination therapy (ACT) of artemether and lumefantrine (Co-artem) has recently replaced chloroquine and fansidar as the first line treatment policy drug in Uganda. It is necessary to develop practical procedures to monitor the likely emergence and spread of artemisinin resistant P. falciparum strains. We have analyzed the genotypes of PfATP6 in parasites from 300 stored filter paper samples from malaria patients who were diagnosed and treated in the years 1999 to 2004 at three field sites in Uganda. This is a period just prior to introduction of Co-artem. In order to develop a simple molecular procedure for mutation detection, regions of PfATP6 encoding protein domains important in artemisinin binding was amplified by nested PCR. Three DNA products, which together contain most of the coding region of amino acids located within the putative active site of pfATP6 were readily amplified. The amplified DNA was digested by restriction enzymes and the fragments sized by agarose gel electrophoresis. For the important codons 260, 263 and 769, methods using engineered restriction sites were employed. We did not find mutations at codons for the key residues Lys 260, Leu263, Gln266, Ser769 and Asn1039. Nucleotide sequencing of pfATPase6 gene DNA from at least 15 clinical isolates confirmed the above findings and suggested that mutations at these amino acid residues have not emerged in our study sites. |
| المشرفين على المادة: | 0 (ATP6 protein, Plasmodium falciparum) 0 (Anti-Infective Agents) 0 (Artemisinins) 0 (Ethanolamines) 0 (Fluorenes) 9RMU91N5K2 (artemisinin) C7D6T3H22J (Artemether) EC 7.2.2.10 (Calcium-Transporting ATPases) F38R0JR742 (Lumefantrine) |
| تواريخ الأحداث: | Date Created: 20140114 Date Completed: 20140128 Latest Revision: 20210915 |
| رمز التحديث: | 20240628 |
| DOI: | 10.4314/thrb.v13i1.58580 |
| PMID: | 24409646 |
| قاعدة البيانات: | MEDLINE |
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