دورية أكاديمية
Up-regulation of neuronal and astrocytic GLT-1 mRNA in the hippocampal CA1 subfield during the induction of brain ischemic tolerance in rats.
العنوان: | Up-regulation of neuronal and astrocytic GLT-1 mRNA in the hippocampal CA1 subfield during the induction of brain ischemic tolerance in rats. |
---|---|
المؤلفون: | Cao M, Zhang M; Hebei Medical University,Shijiazhuang, PR China. Email: hebmuzhangmin@163.com., Zhang LW, Gong JX, Liang CJ, Li WB |
المصدر: | Archives italiennes de biologie [Arch Ital Biol] 2013 Jun; Vol. 151 (2), pp. 43-53. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Universita Degli Studi Di Pisa Country of Publication: Italy NLM ID: 0372441 Publication Model: Print Cited Medium: Print ISSN: 0003-9829 (Print) Linking ISSN: 00039829 NLM ISO Abbreviation: Arch Ital Biol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Pisa : Universita Degli Studi Di Pisa |
مواضيع طبية MeSH: | Brain Ischemia*/pathology , Brain Ischemia*/physiopathology , Brain Ischemia*/prevention & control, Astrocytes/*metabolism , CA1 Region, Hippocampal/*pathology , Excitatory Amino Acid Transporter 2/*genetics , Neurons/*metabolism , RNA, Messenger/*metabolism , Up-Regulation/*physiology, Analysis of Variance ; Animals ; Cell Count ; Disease Models, Animal ; Excitatory Amino Acid Transporter 2/metabolism ; Glial Fibrillary Acidic Protein/metabolism ; Ischemic Preconditioning ; Male ; Rats ; Rats, Wistar ; Time Factors |
مستخلص: | Our previous study showed that 3-min cerebral ischemic preconditioning (CIP) up-regulated the expression of glial glutamate transporter-1 (GLT-1) protein, which protects pyramidal neurons, allowing them to survive an 8-min ischemic insult that usually induces severe delayed neuronal death in the hippocampal CA1 subfield. In the present study, in situ hybridization and immunohistochemistry were used to observe whether GLT-1 mRNA is modulated and whether actrocytes and/or neurons express GLT-1 mRNA during the induction of brain ischemic tolerance. We observed that GLT-1 mRNA is expressed in neurons and astrocytes in the hippocampal CA1 subfield. The expression of GLT-1 mRNA was significantly down-regulated in both neurons and astrocytes after the 8-min lethal ischemic insult. CIP for 3 min increased the expression of GLT-1 mRNA in neurons and astrocytes, and induced the elongation of the astrocytic processes around pyramidal neurons. It may be concluded that CIP performed 2 days before lethal ischemic insult activated astrocytes, which resulted in an increased number of lengthened processes expressing high levels of GLT-1, which protected the neurons and allowed them to survive 8-min ischemic insult that is usually lethal to neurons in the hippocampal CA1 subfield. |
المشرفين على المادة: | 0 (Excitatory Amino Acid Transporter 2) 0 (Glial Fibrillary Acidic Protein) 0 (RNA, Messenger) 0 (Slc1a2 protein, rat) |
تواريخ الأحداث: | Date Created: 20140121 Date Completed: 20140603 Latest Revision: 20181023 |
رمز التحديث: | 20240628 |
DOI: | 10.4449/aib.v151i2.1482 |
PMID: | 24442982 |
قاعدة البيانات: | MEDLINE |
كن أول من يترك تعليقا!