دورية أكاديمية
A unique V-J-C-rearranged gene encodes a gamma protein expressed on the majority of CD3+ T cell receptor-alpha/beta- circulating lymphocytes.
| العنوان: | A unique V-J-C-rearranged gene encodes a gamma protein expressed on the majority of CD3+ T cell receptor-alpha/beta- circulating lymphocytes. |
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| المؤلفون: | Triebel F; Département de Biologie Clinique, Institut Gustave-Roussy, Villejuif, France., Faure F, Graziani M, Jitsukawa S, Lefranc MP, Hercend T |
| المصدر: | The Journal of experimental medicine [J Exp Med] 1988 Feb 01; Vol. 167 (2), pp. 694-9. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 2985109R Publication Model: Print Cited Medium: Print ISSN: 0022-1007 (Print) Linking ISSN: 00221007 NLM ISO Abbreviation: J Exp Med Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Rockefeller University Press |
| مواضيع طبية MeSH: | Genes* , Recombination, Genetic*, Antigens, Differentiation, T-Lymphocyte/*genetics , Receptors, Antigen, T-Cell/*genetics , T-Lymphocytes/*classification, Adult ; Cell Line ; Epitopes/genetics ; Humans ; Mutation ; Nucleic Acid Hybridization ; Phenotype ; Receptors, Antigen, T-Cell/isolation & purification ; T-Lymphocytes/metabolism ; Transcription, Genetic |
| مستخلص: | We have recently described an mAb, anti-Ti gamma A, that recognizes an antigenic determinant carried by a TCR gamma chain. This antibody binds to approximately 3% of human PBLs and delineates a CD2+, CD3+, TCR-alpha/beta-, CD4-, CD8+/-, CD5+, NKH1-, and HLA class II- subset. The present study was designed to identify the gene encoding the Ti gamma A epitope. A first analysis was carried out on a previously characterized TCR gamma + fetal-cloned cell line termed F6C7. It was found that F6C7 cells have one gamma rearrangement on each chromosome: one joins V gamma 3 to J gamma 1, and the second joins V gamma 9 to J gamma P. Because only the latter allele appeared to be transcribed in the F6C7 lymphocytes, these data strongly suggested that anti-Ti gamma A mAb is specific for either a V gamma 9 or a V gamma 9-J gamma P-encoded peptide. To confirm this point, we studied an additional series of 13 randomly selected Ti gamma A+ cloned cells derived from peripheral blood of three distinct adult individuals. Each one of these lymphocytes was shown to both possess and transcribe a V gamma 9-J gamma P-C gamma 1-rearranged gene. It is therefore concluded that a predominant subpopulation of CD3+ TCR-alpha/beta- human circulating T lymphocytes (namely, the subset defined by anti-Ti gamma A mAb) surface expresses a gamma protein with a limited potential of variability from one cell to another. |
| References: | Nature. 1987 Feb 19-25;325(6106):723-6. (PMID: 3102968) Nature. 1986 Jul 10-16;322(6075):184-7. (PMID: 3453106) Eur J Immunol. 1987 Aug;17(8):1209-12. (PMID: 2957218) EMBO J. 1987 Jul;6(7):1945-50. (PMID: 2820713) J Exp Med. 1987 Oct 1;166(4):1192-7. (PMID: 2443600) |
| المشرفين على المادة: | 0 (Antigens, Differentiation, T-Lymphocyte) 0 (Epitopes) 0 (Receptors, Antigen, T-Cell) |
| تواريخ الأحداث: | Date Created: 19880201 Date Completed: 19880413 Latest Revision: 20200304 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC2188842 |
| DOI: | 10.1084/jem.167.2.694 |
| PMID: | 2450164 |
| قاعدة البيانات: | MEDLINE |
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