دورية أكاديمية
Effect of calcium channel modulators on isolated endothelial cells.
| العنوان: | Effect of calcium channel modulators on isolated endothelial cells. |
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| المؤلفون: | Whitmer KR; Department of Pharmacology, University of Cincinnati College of Medicine, Ohio., Williams-Lawson JS, Highsmith RF, Schwartz A |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1988 Jul 29; Vol. 154 (2), pp. 591-605. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print Cited Medium: Print ISSN: 0006-291X (Print) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Calcium/*metabolism , Endothelium/*metabolism , Ion Channels/*drug effects, Adenosine Triphosphate/pharmacology ; Animals ; Benzofurans ; Biological Products/metabolism ; Bradykinin/pharmacology ; Carbachol/pharmacology ; Cattle ; Diltiazem/pharmacology ; Endothelium/drug effects ; Fura-2 ; Isradipine ; Nitric Oxide ; Oxadiazoles/pharmacology ; Thrombin/pharmacology ; Verapamil/pharmacology |
| مستخلص: | Indirect evidence, using organic calcium channel modulators suggests that calcium channels exist in endothelial cells. Using freshly prepared and cultured bovine aortic endothelial cells, we have studied the effect of calcium channel modulators on Fura-2 fluorescence and have examined the binding of the dihydropyridine, (+)[3H]PN200-110. In both isolated primary and cultured cells, external calcium (0.5-2 mM) and bradykinin (10(-8) M) increased the intracellular calcium concentration. In cultured cells, the increase in calcium was not significantly attenuated by preincubation with nitrendipine (10(-8) M) or d-cis-diltiazem (10(-6) M). The calcium agonists (-)Bay k8644 and (+)202-791 had no effect on intracellular calcium concentration, but other agonists including ATP (10(-4) M) and thrombin (1.5 micrograms/ml) significantly increased the calcium concentration. Competition binding studies with (+)[3H]PN200-110 indicated specific binding of this ligand with a KD of 57 nM and a Bmax of 2.1 pmol/10(6) cells. While these data do not provide convincing evidence for the existence of calcium channels in cultured or fresh bovine aortic endothelial cells, explanations may yet reconcile our observations with the presence of calcium channels in these cells. |
| معلومات مُعتمدة: | HL 07382 United States HL NHLBI NIH HHS; HL 31543 United States HL NHLBI NIH HHS |
| المشرفين على المادة: | 0 (Benzofurans) 0 (Biological Products) 0 (Ion Channels) 0 (Oxadiazoles) 31C4KY9ESH (Nitric Oxide) 8L70Q75FXE (Adenosine Triphosphate) 8Y164V895Y (Carbachol) CJ0O37KU29 (Verapamil) EC 3.4.21.5 (Thrombin) EE92BBP03H (Diltiazem) S8TIM42R2W (Bradykinin) SY7Q814VUP (Calcium) TSN3DL106G (Fura-2) YO1UK1S598 (Isradipine) |
| تواريخ الأحداث: | Date Created: 19880729 Date Completed: 19880902 Latest Revision: 20190612 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/0006-291x(88)90181-7 |
| PMID: | 2456759 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0006-291X |
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| DOI: | 10.1016/0006-291x(88)90181-7 |