دورية أكاديمية
Identification of an estrogen receptor gene in the natural freshwater snail Bithynia tentaculata.
| العنوان: | Identification of an estrogen receptor gene in the natural freshwater snail Bithynia tentaculata. |
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| المؤلفون: | Hultin CL; Centre for Environmental and Climate Research (CEC), Lund University, Sölvegatan 37, SE-223 62 Lund, Sweden. Electronic address: cecilia.hultin@cec.lu.se., Hallgren P; Aquatic Ecology, Department of Biology, Lund University, Sölvegatan 37, SE-223 62 Lund, Sweden., Persson A; Aquatic Ecology, Department of Biology, Lund University, Sölvegatan 37, SE-223 62 Lund, Sweden., Hansson MC; Centre for Environmental and Climate Research (CEC), Lund University, Sölvegatan 37, SE-223 62 Lund, Sweden. |
| المصدر: | Gene [Gene] 2014 Apr 25; Vol. 540 (1), pp. 26-31. Date of Electronic Publication: 2014 Feb 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland, 1976- |
| مواضيع طبية MeSH: | Receptors, Estrogen/*genetics , Snails/*genetics, Amino Acid Sequence ; Animals ; Aquatic Organisms/genetics ; Conserved Sequence ; Estrogens/pharmacology ; Ethinyl Estradiol/pharmacology ; Female ; Fresh Water ; Gene Expression/drug effects ; Molecular Sequence Data ; Phylogeny ; Receptors, Estrogen/metabolism ; Snails/metabolism |
| مستخلص: | Mollusks have received increasing interest in ecotoxicological studies but so far the available scientific analyses of how their genes are affected by anthropogenic pollutants are scarce. The focus of this study is to identify an estrogen receptor (er) gene in the common prosobranch snail Bithynia tentaculata and to test a hypothesis that 17α-Ethinylestradiol (EE2) will modulate er gene expression after short-term exposure. We set up exposure experiments with a total of 144 snails, which were collected from a natural population in southern Sweden. Snails were exposed to either 10ng/L or 100ng/L EE2 during 24h and/or 72h. From the isolated B. tentaculata RNA we successfully identified and characterized a novel er gene and phylogenetic analyses strongly indicate that the Bithynia er gene is an ortholog to the human ERα (ESR1, NR3A1). We found a significant interaction between EE2-dose and exposure duration on the er's gene expression (Two-way ANOVA; p=0.04). We also found a significant difference in the gene expression of the er when comparing the control and 100ng/L treatment groups after 72h in female snails (One-way ANOVA; p=0.047). The results from this study should be useful for future field-related studies of estrogen receptors in natural populations of mollusks. (Copyright © 2014 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 17α-Ethinylestradiol; Bithynia; Estrogen receptor; Mollusk |
| المشرفين على المادة: | 0 (Estrogens) 0 (Receptors, Estrogen) 423D2T571U (Ethinyl Estradiol) |
| تواريخ الأحداث: | Date Created: 20140304 Date Completed: 20140520 Latest Revision: 20140317 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.gene.2014.02.039 |
| PMID: | 24583164 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0038 |
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| DOI: | 10.1016/j.gene.2014.02.039 |