دورية أكاديمية
أعرض في EDS

Molecular cloning of Ra-sHSPI, a novel member of the HSP20 family from Rhipicephalus annulatus salivary glands.

التفاصيل البيبلوغرافية
العنوان: Molecular cloning of Ra-sHSPI, a novel member of the HSP20 family from Rhipicephalus annulatus salivary glands.
المؤلفون: Hussein NA; Molecular Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Cairo, Egypt., Shahein YE; Molecular Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Cairo, Egypt. Electronic address: yassershahein_nrc@yahoo.com., El-Hakim AE; Molecular Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Cairo, Egypt., Abouelella AM; Radiation Biology Department, National Centre for Radiation Research and Technology (NCRRT), Nasr City, Cairo, Egypt., Guneidy RA; Molecular Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Cairo, Egypt., Hamed RR; Molecular Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Cairo, Egypt.
المصدر: International journal of biological macromolecules [Int J Biol Macromol] 2014 Jun; Vol. 67, pp. 7-15. Date of Electronic Publication: 2014 Mar 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Guildford, Eng., IPC Science and Technology Press.
مواضيع طبية MeSH: Cloning, Molecular*, HSP20 Heat-Shock Proteins/*genetics , Rhipicephalus/*genetics, Animals ; Cattle/genetics ; Cattle/parasitology ; DNA, Complementary/genetics ; Salivary Glands ; Sequence Analysis, DNA
مستخلص: Infestation of cattle by ticks of Rhipicephalus spp. results in severe veterinary and economical losses. Identification of novel proteins from tick salivary glands will enhance our understanding of several aspects of tick physiology and will aid in the development of anti-tick vaccines. Small heat shock proteins (HSPs) have important roles in infection and immunity, especially between invertebrate vectors and mammalian hosts while initially performing their molecular chaperone activity. Here, we report the identification of a small HSP gene from the salivary glands of Rhipicephalus annulatus ticks through immunoscreening of the corresponding cDNA expression library. The identified cDNA contained a 742bp sequence with 543bp open reading frame. It was subsequently cloned, expressed and successfully purified under both native and denaturing conditions. Sequence analysis and functional investigations showed that the protein belongs to the HSP20 family, hence the annotated name Ra-sHSPI. Indeed, recombinant Ra-sHSPI showed two typical in vitro activities of holdase chaperones, including thermal protection of bacterial cellular extracts and the recombinant HindIII at elevated temperatures. Moreover, the recombinant Ra-sHSPI showed strong immunogenic effect in animal model. These results pave the way toward further investigation of Ra-sHSPI role in ticks feeding and its potential use as protective antigen.
(Copyright © 2014 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Cattle ticks; Rhipicephalus annulatus; Salivary glands; Thermal stability; sHSP
المشرفين على المادة: 0 (DNA, Complementary)
0 (HSP20 Heat-Shock Proteins)
تواريخ الأحداث: Date Created: 20140315 Date Completed: 20150105 Latest Revision: 20140521
رمز التحديث: 20240628
DOI: 10.1016/j.ijbiomac.2014.02.057
PMID: 24625965
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0003
DOI:10.1016/j.ijbiomac.2014.02.057