دورية أكاديمية
The AAT1 locus is critical for the biosynthesis of esters contributing to 'ripe apple' flavour in 'Royal Gala' and 'Granny Smith' apples.
| العنوان: | The AAT1 locus is critical for the biosynthesis of esters contributing to 'ripe apple' flavour in 'Royal Gala' and 'Granny Smith' apples. |
|---|---|
| المؤلفون: | Souleyre EJ; The New Zealand Institute for Plant and Food Research Limited (PFR), Private Bag 92169, Victoria Street West, Auckland, 1025, New Zealand., Chagné D, Chen X, Tomes S, Turner RM, Wang MY, Maddumage R, Hunt MB, Winz RA, Wiedow C, Hamiaux C, Gardiner SE, Rowan DD, Atkinson RG |
| المصدر: | The Plant journal : for cell and molecular biology [Plant J] 2014 Jun; Vol. 78 (6), pp. 903-15. Date of Electronic Publication: 2014 May 23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology Country of Publication: England NLM ID: 9207397 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-313X (Electronic) Linking ISSN: 09607412 NLM ISO Abbreviation: Plant J Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford : Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology, c1991- |
| مواضيع طبية MeSH: | Quantitative Trait Loci*, Acetyltransferases/*genetics , Esters/*metabolism , Malus/*genetics , Plant Proteins/*genetics, Acetyltransferases/metabolism ; Acetyltransferases/physiology ; Chromosome Mapping ; Down-Regulation ; Genetic Association Studies ; Genetic Linkage ; Genetic Variation ; Malus/metabolism ; Molecular Sequence Data ; Plant Proteins/metabolism ; Plant Proteins/physiology ; Plants, Genetically Modified/metabolism |
| مستخلص: | The 'fruity' attributes of ripe apples (Malus × domestica) arise from our perception of a combination of volatile ester compounds. Phenotypic variability in ester production was investigated using a segregating population from a 'Royal Gala' (RG; high ester production) × 'Granny Smith' (GS; low ester production) cross, as well as in transgenic RG plants in which expression of the alcohol acyl transferase 1 (AAT1) gene was reduced. In the RG × GS population, 46 quantitative trait loci (QTLs) for the production of esters and alcohols were identified on 15 linkage groups (LGs). The major QTL for 35 individual compounds was positioned on LG2 and co-located with AAT1. Multiple AAT1 gene variants were identified in RG and GS, but only two (AAT1-RGa and AAT1-GSa) were functional. AAT1-RGa and AAT1-GSa were both highly expressed in the cortex and skin of ripe fruit, but AAT1 protein was observed mainly in the skin. Transgenic RG specifically reduced in AAT1 expression showed reduced levels of most key esters in ripe fruit. Differences in the ripe fruit aroma could be perceived by sensory analysis. The transgenic lines also showed altered ratios of biosynthetic precursor alcohols and aldehydes, and expression of a number of ester biosynthetic genes increased, presumably in response to the increased substrate pool. These results indicate that the AAT1 locus is critical for the biosynthesis of esters contributing to a 'ripe apple' flavour. (© 2014 The Authors The Plant Journal © 2014 John Wiley & Sons Ltd.) |
| فهرسة مساهمة: | Keywords: Malus domestica; apple; aroma; ester; flavour; fruit; quantitative trait loci; transgenic; volatile |
| سلسلة جزيئية: | GENBANK KC291129; KC291130; KC291131; KC291132; KC291133; KC291134; KC291135 |
| المشرفين على المادة: | 0 (Esters) 0 (Plant Proteins) EC 2.3.1.- (Acetyltransferases) |
| تواريخ الأحداث: | Date Created: 20140326 Date Completed: 20150224 Latest Revision: 20140612 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1111/tpj.12518 |
| PMID: | 24661745 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1365-313X |
|---|---|
| DOI: | 10.1111/tpj.12518 |