دورية أكاديمية
A high-grain diet alters the omasal epithelial structure and expression of tight junction proteins in a goat model.
العنوان: | A high-grain diet alters the omasal epithelial structure and expression of tight junction proteins in a goat model. |
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المؤلفون: | Liu JH; Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China., Xu TT; Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China., Zhu WY; Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China., Mao SY; Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China. Electronic address: maoshengyong@163.com. |
المصدر: | Veterinary journal (London, England : 1997) [Vet J] 2014 Jul; Vol. 201 (1), pp. 95-100. Date of Electronic Publication: 2014 Mar 31. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Balliere Tindall Country of Publication: England NLM ID: 9706281 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2971 (Electronic) Linking ISSN: 10900233 NLM ISO Abbreviation: Vet J Subsets: MEDLINE |
أسماء مطبوعة: | Publication: London : Balliere Tindall Original Publication: London : Ballière Tindall, c1997- |
مواضيع طبية MeSH: | Gene Expression Regulation*, Diet/*veterinary , Edible Grain/*metabolism , Epithelial Cells/*cytology , Goats/*physiology , Omasum/*cytology , Tight Junction Proteins/*genetics, Animals ; Blotting, Western/veterinary ; Epithelial Cells/metabolism ; Goats/genetics ; Male ; Omasum/metabolism ; Random Allocation ; Tight Junction Proteins/metabolism |
مستخلص: | The omasal epithelial barrier plays important roles in maintaining nutrient absorption and immune homeostasis in ruminants. However, little information is currently available about the changes in omasal epithelial barrier function at the structural and molecular levels during feeding of a high-grain (HG) diet. Ten male goats were randomly assigned to two groups, fed either a hay diet (0% grain; n = 5) or HG diet (65% grain; n = 5). Changes in omasal epithelial structure and expression of tight junction (TJ) proteins were determined via electron microscopy and Western blot analysis. After 7 weeks on each diet, omasal contents in the HG group showed significantly lower pH (P <0.001) and significantly higher concentrations of free lipopolysaccharides (LPS; P = 0.001) than the hay group. The goats fed a HG diet showed profound alterations in omasal epithelial structure and TJ proteins, corresponding to depression of thickness of total epithelia, stratum granulosum, and the sum of the stratum spinosum and stratum basale, marked epithelial cellular damage, erosion of intercellular junctions and down-regulation in expression of the TJ proteins, claudin-4 and occludin. The study demonstrates that feeding a HG diet is associated with omasal epithelial cellular damage and changes in expression of TJ proteins. These research findings provide an insight into the possible significance of diet on the omasal epithelial barrier in ruminants. (Copyright © 2014 Elsevier Ltd. All rights reserved.) |
فهرسة مساهمة: | Keywords: Goat; High-grain diet; Lipopolysaccharide; Omasal epithelium; Tight junction protein |
المشرفين على المادة: | 0 (Tight Junction Proteins) |
تواريخ الأحداث: | Date Created: 20140608 Date Completed: 20150410 Latest Revision: 20151119 |
رمز التحديث: | 20231215 |
DOI: | 10.1016/j.tvjl.2014.03.025 |
PMID: | 24906500 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1532-2971 |
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DOI: | 10.1016/j.tvjl.2014.03.025 |