دورية أكاديمية
Fusarium graminearum PKS14 is involved in orsellinic acid and orcinol synthesis.
| العنوان: | Fusarium graminearum PKS14 is involved in orsellinic acid and orcinol synthesis. |
|---|---|
| المؤلفون: | Jørgensen SH; Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, DK-9000 Aalborg, Denmark., Frandsen RJ; Department of Systems Biology, Technical University of Denmark, Søltofts Plads, DK-2800 Kgs. Lyngby, Denmark., Nielsen KF; Department of Systems Biology, Technical University of Denmark, Søltofts Plads, DK-2800 Kgs. Lyngby, Denmark., Lysøe E; Department of Plant Health and Plant Protection, Bioforsk-Norwegian Institute of Agricultural and Environmental Research, 1432 Ås, Norway., Sondergaard TE; Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, DK-9000 Aalborg, Denmark., Wimmer R; Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, DK-9000 Aalborg, Denmark., Giese H; Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, DK-9000 Aalborg, Denmark., Sørensen JL; Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, DK-9000 Aalborg, Denmark. Electronic address: jls@bio.aau.dk. |
| المصدر: | Fungal genetics and biology : FG & B [Fungal Genet Biol] 2014 Sep; Vol. 70, pp. 24-31. Date of Electronic Publication: 2014 Jul 08. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 9607601 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0937 (Electronic) Linking ISSN: 10871845 NLM ISO Abbreviation: Fungal Genet Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Orlando, FL : Academic Press, c1996- |
| مواضيع طبية MeSH: | Fungal Proteins/*metabolism , Fusarium/*metabolism , Polyketide Synthases/*metabolism , Resorcinols/*metabolism, Decarboxylation ; Fungal Proteins/genetics ; Fusarium/genetics ; Multigene Family ; Mutation ; Phylogeny ; Polyketide Synthases/genetics |
| مستخلص: | The available genome sequences show that the number of secondary metabolite genes in filamentous fungi vastly exceeds the number of known products. This is also true for the global plant pathogenic fungus Fusarium graminearum, which contains 15 polyketide synthase (PKS) genes, of which only 6 have been linked to products. To help remedy this, we focused on PKS14, which has only been shown to be expressed during plant infections or when cultivated on rice or corn meal (RM) based media. To enhance the production of the resulting product we introduced a constitutive promoter in front of PKS14 and cultivated two of the resulting mutants on RM medium. This led to the production of two compounds, which were only detected in the PKS14 overexpressing mutants and not in the wild type or PKS14 deletion mutants. The two compounds were tentatively identified as orsellinic acid and orcinol by comparing spectroscopic data (mass spectroscopy and chromatography) to authentic standards. NMR analysis of putative orcinol isolated from the PKS14 overexpressing mutant supported our identification. Orcinol and orsellinic acid, not previously detected in Fusarium, have primarily been detected in lichen fungi. Orsellinic acid is hypothesized to be the PKS release product which is transformed to orcinol through decarboxylation. Phylogenetic analyses of PKSs placed PKS14 in a subclade of known OA synthases. Expression analysis by microarray of 55 experiments identified seven genes near PKS14 that were expressed in a similar manner. One of the seven genes encodes a predicted carboxylase, which could be responsible for transforming orsellinic acid to orcinol. (Copyright © 2014 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Fusarium; Overexpression; Polyketide synthase 14; Regulation; Secondary metabolites |
| المشرفين على المادة: | 0 (Fungal Proteins) 0 (Resorcinols) 11XLA0494B (orsellinic acid) 534PMB3438 (orcinol) 79956-01-7 (Polyketide Synthases) |
| تواريخ الأحداث: | Date Created: 20140711 Date Completed: 20150416 Latest Revision: 20140906 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.fgb.2014.06.008 |
| PMID: | 25011010 |
| قاعدة البيانات: | MEDLINE |
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