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Characterization of the rumen microbiome of Indian Kankrej cattle (Bos indicus) adapted to different forage diet.

التفاصيل البيبلوغرافية
العنوان: Characterization of the rumen microbiome of Indian Kankrej cattle (Bos indicus) adapted to different forage diet.
المؤلفون: Patel V; Department of Animal Biotechnology, College of Veterinary, Science and Animal Husbandry, Anand Agricultural University (AAU), Anand, 388 001, Gujarat, India., Patel AK, Parmar NR, Patel AB, Reddy B, Joshi CG
المصدر: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2014 Dec; Vol. 98 (23), pp. 9749-61. Date of Electronic Publication: 2014 Oct 31.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin ; New York : Springer International, c1984-
مواضيع طبية MeSH: Animal Feed* , Biota*, Bacteria/*classification , Bacteria/*genetics , Diet/*methods , Rumen/*microbiology, Animals ; Cattle ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; Metabolic Networks and Pathways/genetics ; Metagenomics ; Molecular Sequence Data ; Sequence Analysis, DNA
مستخلص: Present study described rumen microbiome of Indian cattle (Kankrej breed) to better understand the microbial diversity and largely unknown functional capacity of the rumen microbiome under different dietary treatments. Kankrej cattle were gradually adapted to a high-forage diet (four animals with dry forage and four with green forage) containing 50 % (K1), 75 % (K2) to 100 % (K3) forage, and remaining concentrate diet, each for 6 weeks followed by analysis of rumen fiber adherent and fiber-free metagenomic community by shotgun sequencing using ion torrent PGM platform and EBI-metagenomics annotation pipeline. Taxonomic analysis indicated that rumen microbiome was dominated by Bacteroidetes followed by Firmicutes, Fibrobacter, Proteobacteria, and Tenericutes. Functional analysis based on gene ontology classified all reads in total 157 categories based on their functional role in biological, molecular, and cellular component with abundance of genes associated with hydrolase activity, membrane, transport, transferase, and different metabolism (such as carbohydrate and protein). Statistical analysis using STAMP revealed significant differences (P < 0.05) between solid and liquid fraction of rumen (in 65 categories), between all three treatments (in 56 categories), and between green and dry roughage (17 categories). Diet treatment also exerted significant difference in environmental gene tags (EGTs) involved in metabolic pathways for production of volatile fatty acids. EGTs for butyrate production were abundant in K2, whereas EGTs for propionate production was abundant during K1. Principal component analysis also demonstrated that diet proportion, fraction of rumen, and type of forage affected rumen microbiome at taxonomic as well as functional level.
المشرفين على المادة: 0 (DNA, Bacterial)
تواريخ الأحداث: Date Created: 20141101 Date Completed: 20150624 Latest Revision: 20141114
رمز التحديث: 20240628
DOI: 10.1007/s00253-014-6153-1
PMID: 25359471
قاعدة البيانات: MEDLINE
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