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A molecular toggle after exocytosis sequesters the presynaptic syntaxin1a molecules involved in prior vesicle fusion.

التفاصيل البيبلوغرافية
العنوان: A molecular toggle after exocytosis sequesters the presynaptic syntaxin1a molecules involved in prior vesicle fusion.
المؤلفون: Kavanagh DM; 1] Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot Watt University, Edinburgh EH14 4AS, UK [2] Edinburgh Super-Resolution Imaging Consortium, www.esric.org., Smyth AM; 1] Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot Watt University, Edinburgh EH14 4AS, UK [2] Edinburgh Super-Resolution Imaging Consortium, www.esric.org [3] Centre for Integrative Physiology, University of Edinburgh, George Square, Edinburgh EH8 9XD, UK., Martin KJ; 1] Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot Watt University, Edinburgh EH14 4AS, UK [2] Edinburgh Super-Resolution Imaging Consortium, www.esric.org., Dun A; 1] Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot Watt University, Edinburgh EH14 4AS, UK [2] Edinburgh Super-Resolution Imaging Consortium, www.esric.org., Brown ER; 1] Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot Watt University, Edinburgh EH14 4AS, UK [2] Edinburgh Super-Resolution Imaging Consortium, www.esric.org., Gordon S; Centre for Integrative Physiology, University of Edinburgh, George Square, Edinburgh EH8 9XD, UK., Smillie KJ; Centre for Integrative Physiology, University of Edinburgh, George Square, Edinburgh EH8 9XD, UK., Chamberlain LH; Strathclyde Institute of Pharmacy and Biomedical Sciences, 161 Cathedral Street, Glasgow G4 0RE, UK., Wilson RS; 1] Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot Watt University, Edinburgh EH14 4AS, UK [2] Edinburgh Super-Resolution Imaging Consortium, www.esric.org., Yang L; 1] Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot Watt University, Edinburgh EH14 4AS, UK [2] Edinburgh Super-Resolution Imaging Consortium, www.esric.org., Lu W; 1] Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot Watt University, Edinburgh EH14 4AS, UK [2] Edinburgh Super-Resolution Imaging Consortium, www.esric.org., Cousin MA; Centre for Integrative Physiology, University of Edinburgh, George Square, Edinburgh EH8 9XD, UK., Rickman C; 1] Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot Watt University, Edinburgh EH14 4AS, UK [2] Edinburgh Super-Resolution Imaging Consortium, www.esric.org., Duncan RR; 1] Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot Watt University, Edinburgh EH14 4AS, UK [2] Edinburgh Super-Resolution Imaging Consortium, www.esric.org.
المصدر: Nature communications [Nat Commun] 2014 Dec 17; Vol. 5, pp. 5774. Date of Electronic Publication: 2014 Dec 17.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Exocytosis/*genetics , Munc18 Proteins/*metabolism , Synapses/*metabolism , Synaptic Vesicles/*metabolism , Syntaxin 1/*metabolism, Animals ; Botulinum Toxins/pharmacology ; Botulinum Toxins, Type A/pharmacology ; Cerebral Cortex/cytology ; Cerebral Cortex/drug effects ; Cerebral Cortex/metabolism ; Embryo, Mammalian ; Gene Expression Regulation ; Genes, Reporter ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Membrane Fusion ; Molecular Imaging ; Munc18 Proteins/genetics ; Neurons/cytology ; Neurons/drug effects ; Neurons/metabolism ; Primary Cell Culture ; Protein Binding ; Protein Transport ; Rats ; Rats, Sprague-Dawley ; SNARE Proteins/genetics ; SNARE Proteins/metabolism ; Synapses/drug effects ; Synapses/ultrastructure ; Synaptic Transmission ; Synaptic Vesicles/drug effects ; Synaptic Vesicles/ultrastructure ; Syntaxin 1/genetics ; Red Fluorescent Protein
مستخلص: Neuronal synapses are among the most scrutinized of cellular systems, serving as a model for all membrane trafficking studies. Despite this, synaptic biology has proven difficult to interrogate directly in situ due to the small size and dynamic nature of central synapses and the molecules within them. Here we determine the spatial and temporal interaction status of presynaptic proteins, imaging large cohorts of single molecules inside active synapses. Measuring rapid interaction dynamics during synaptic depolarization identified the small number of syntaxin1a and munc18-1 protein molecules required to support synaptic vesicle exocytosis. After vesicle fusion and subsequent SNARE complex disassembly, a prompt switch in syntaxin1a and munc18-1-binding mode, regulated by charge alteration on the syntaxin1a N-terminal, sequesters monomeric syntaxin1a from other disassembled fusion complex components, preventing ectopic SNARE complex formation, readying the synapse for subsequent rounds of neurotransmission.
References: J Neurosci. 2000 Jun 15;20(12):4535-44. (PMID: 10844023)
Curr Top Membr. 2013;72:89-120. (PMID: 24210428)
Nat Neurosci. 2001 Feb;4(2):129-36. (PMID: 11175872)
Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):8065-70. (PMID: 11427709)
Neuron. 2001 Aug 30;31(4):581-91. (PMID: 11545717)
J Cell Sci. 2001 Sep;114(Pt 18):3323-32. (PMID: 11591820)
Science. 2002 Feb 8;295(5557):1083-6. (PMID: 11834838)
Methods. 2003 Feb;29(2):153-66. (PMID: 12606221)
J Microsc. 2004 Jul;215(Pt 1):1-12. (PMID: 15230870)
Cell. 1990 May 18;61(4):709-21. (PMID: 2111733)
Nature. 1993 Mar 25;362(6418):318-24. (PMID: 8455717)
Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1445-9. (PMID: 8108429)
EMBO J. 1994 Nov 1;13(21):5051-61. (PMID: 7957071)
J Biol Chem. 1995 Jul 14;270(28):16955-61. (PMID: 7622514)
Cell. 1996 Apr 5;85(1):83-94. (PMID: 8620540)
Neuron. 1998 Jul;21(1):123-36. (PMID: 9697857)
Nature. 1998 Sep 24;395(6700):347-53. (PMID: 9759724)
J Cell Sci. 1999 Jun;112 ( Pt 12):1865-77. (PMID: 10341206)
EMBO J. 1999 Aug 16;18(16):4372-82. (PMID: 10449403)
EMBO Rep. 2004 Nov;5(11):1090-5. (PMID: 15486565)
J Biol Chem. 2004 Dec 31;279(53):55924-36. (PMID: 15489225)
Science. 2006 Sep 15;313(5793):1642-5. (PMID: 16902090)
Nat Methods. 2006 Oct;3(10):793-5. (PMID: 16896339)
Biophys J. 2006 Dec 1;91(11):4258-72. (PMID: 16980368)
Cell. 2007 Jan 12;128(1):183-95. (PMID: 17218264)
Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2697-702. (PMID: 17301226)
J Biol Chem. 2007 Apr 20;282(16):12097-103. (PMID: 17264080)
J Neurosci. 2007 Aug 8;27(32):8676-86. (PMID: 17687045)
J Cell Sci. 2007 Dec 15;120(Pt 24):4407-15. (PMID: 18057031)
Nat Methods. 2008 Feb;5(2):155-7. (PMID: 18193054)
Mol Biol Cell. 2008 Feb;19(2):485-97. (PMID: 18003982)
Biochem J. 2008 Aug 1;413(3):479-91. (PMID: 18452404)
Science. 2008 Sep 12;321(5895):1507-10. (PMID: 18703708)
Science. 2009 Jan 23;323(5913):474-7. (PMID: 19164740)
J Cell Biol. 2009 Mar 9;184(5):751-64. (PMID: 19255244)
Cell. 2009 Sep 4;138(5):935-46. (PMID: 19716167)
Nat Methods. 2014 Feb;11(2):121-2. (PMID: 24481215)
J Cell Biol. 2014 Mar 3;204(5):759-75. (PMID: 24590174)
Science. 2014 May 30;344(6187):1023-8. (PMID: 24876496)
J Biol Chem. 2010 Feb 5;285(6):3965-72. (PMID: 19748891)
Traffic. 2010 Mar;11(3):394-404. (PMID: 20002656)
J Biol Chem. 2010 Apr 30;285(18):13535-41. (PMID: 20093362)
Science. 2010 Oct 22;330(6003):502-5. (PMID: 20847232)
J Struct Biol. 2010 Dec;172(3):233-43. (PMID: 20599512)
J Biol Chem. 2010 Dec 3;285(49):38141-8. (PMID: 20801887)
Cell Mol Neurobiol. 2010 Nov;30(8):1321-6. (PMID: 21046449)
Nat Protoc. 2011 Jul;6(7):991-1009. (PMID: 21720313)
EMBO J. 2012 May 2;31(9):2156-68. (PMID: 22446389)
PLoS One. 2012;7(11):e49514. (PMID: 23166692)
Nat Commun. 2013;4:1472. (PMID: 23403573)
J Biol Chem. 2013 Feb 15;288(7):5102-13. (PMID: 23223447)
J Neurosci. 2013 Mar 27;33(13):5507-23. (PMID: 23536066)
Proc Natl Acad Sci U S A. 2013 Jul 30;110(31):12637-42. (PMID: 23858467)
Science. 2001 Feb 2;291(5505):875-8. (PMID: 11157167)
معلومات مُعتمدة: WT092478MA United Kingdom Wellcome Trust; MR/K018639/1 United Kingdom Medical Research Council; G1002117 United Kingdom Medical Research Council; MR/K01563X/1 United Kingdom MRC_ Medical Research Council; G0901607 United Kingdom MRC_ Medical Research Council; 074146 United Kingdom Wellcome Trust
المشرفين على المادة: 0 (Luminescent Proteins)
0 (Munc18 Proteins)
0 (SNARE Proteins)
0 (Stxbp1 protein, rat)
0 (Syntaxin 1)
0 (enhanced green fluorescent protein)
147336-22-9 (Green Fluorescent Proteins)
EC 3.4.24.69 (Botulinum Toxins)
EC 3.4.24.69 (Botulinum Toxins, Type A)
FPM7829VMX (botulinum toxin type C)
تواريخ الأحداث: Date Created: 20141218 Date Completed: 20151002 Latest Revision: 20231213
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC4284649
DOI: 10.1038/ncomms6774
PMID: 25517944
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/ncomms6774