دورية أكاديمية
Pannexin-1 channels do not regulate α1-adrenoceptor-mediated vasoconstriction in resistance arteries.
| العنوان: | Pannexin-1 channels do not regulate α1-adrenoceptor-mediated vasoconstriction in resistance arteries. |
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| المؤلفون: | Angus JA; Cardiovascular Therapeutics Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Victoria 3010, Australia. Electronic address: jamesaa@unimelb.edu.au., Betrie AH; Cardiovascular Therapeutics Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Victoria 3010, Australia. Electronic address: a.betrie@student.unimelb.edu.au., Wright CE; Cardiovascular Therapeutics Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Victoria 3010, Australia. Electronic address: cewright@unimelb.edu.au. |
| المصدر: | European journal of pharmacology [Eur J Pharmacol] 2015 Mar 05; Vol. 750, pp. 43-51. Date of Electronic Publication: 2015 Jan 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 1254354 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0712 (Electronic) Linking ISSN: 00142999 NLM ISO Abbreviation: Eur J Pharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2005- : Amsterdam : Elsevier Science Original Publication: Amsterdam, North Holland Pub. Co. |
| مواضيع طبية MeSH: | Vasoconstriction*/drug effects, Arteries/*physiology , Receptors, Adrenergic, alpha-1/*metabolism, Animals ; Arteries/drug effects ; Arteries/innervation ; Arteries/metabolism ; Carbenoxolone/pharmacology ; Connexins/metabolism ; Dose-Response Relationship, Drug ; Mefloquine/pharmacology ; Mesenteric Arteries/drug effects ; Mesenteric Arteries/innervation ; Mesenteric Arteries/metabolism ; Mesenteric Arteries/physiology ; Mice ; Nerve Tissue Proteins/metabolism ; Phenylephrine/pharmacology ; Rats ; Vasoconstrictor Agents/pharmacology |
| مستخلص: | Recent reports have provided evidence for a new concept that in small resistance arteries α1D-adrenoceptor-mediated contraction is intimately linked to pannexin-1 (Px1) hemichannels that open to allow the release of ATP, from the smooth muscle effector cell, that acts back on P2Y purinoceptors to cause contraction. This concept mainly relied on using mefloquine 10-20μM as a putative selective Px1 channel-blocking agent to completely inhibit the contraction to phenylephrine, but not K(+) 40mM. Lower concentrations of mefloquine had no effect. The purpose of the present study was to explore the specificity of mefloquine for Px1 channels and the role of these channels in small artery contraction. In mouse and rat isolated small resistance arteries, either pressurised or set up for wire myography, the effects of mefloquine on contractions to K(+), phenylephrine and a range of vasoconstrictor agents were assessed and compared with the Px1 channel inhibitor carbenoxolone. Mefloquine had a wide range of inhibitory actions at 10-20μM, some 200-fold above the concentrations previously shown to inhibit expressed Px1 channel activity. Mefloquine 3-10μM inhibited phenylephrine, U46619, vasopressin, endothelin-1, sympathetic nerve stimulation and K(+) 40mM-mediated contractions in rat and mouse small mesenteric, and mouse thoracodorsal, arteries. Carbenoxolone 1-100μM did not inhibit the contractile responses to these agents in small resistance arteries. The present study demonstrates that in small resistance arteries there is no evidence that Px1 channels releasing ATP have any role in the constrictor actions of α1-adrenoceptor activation. (Copyright © 2015 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: ATP release; Carbenoxolone; Carbenoxolone disodium salt (PubChem CID: 6419769); Mefloquine; Mefloquine hydrochloride (PubChem CID: 65329); Pannexin-1 channels; Phenylephrine hydrochloride (PubChem CID: 5284443); Vasoconstriction; α(1)-adrenoceptors |
| المشرفين على المادة: | 0 (Connexins) 0 (Nerve Tissue Proteins) 0 (Panx1 protein, mouse) 0 (Receptors, Adrenergic, alpha-1) 0 (Vasoconstrictor Agents) 1WS297W6MV (Phenylephrine) MM6384NG73 (Carbenoxolone) TML814419R (Mefloquine) |
| تواريخ الأحداث: | Date Created: 20150201 Date Completed: 20151124 Latest Revision: 20150302 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.ejphar.2015.01.024 |
| PMID: | 25637780 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0712 |
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| DOI: | 10.1016/j.ejphar.2015.01.024 |