دورية أكاديمية
Supercooling preservation and transplantation of the rat liver.
| العنوان: | Supercooling preservation and transplantation of the rat liver. |
|---|---|
| المؤلفون: | Bruinsma BG; 1] Department of Surgery, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA. [2] Department of Surgery (Surgical Laboratory), Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands., Berendsen TA; Department of Surgery, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA., Izamis ML; Department of Surgery, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA., Yeh H; Transplant Center, Massachusetts General Hospital, Boston, Massachusetts, USA., Yarmush ML; 1] Department of Surgery, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA. [2] Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey, USA., Uygun K; Department of Surgery, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA. |
| المصدر: | Nature protocols [Nat Protoc] 2015 Mar; Vol. 10 (3), pp. 484-94. Date of Electronic Publication: 2015 Feb 18. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101284307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1750-2799 (Electronic) Linking ISSN: 17502799 NLM ISO Abbreviation: Nat Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London, UK : Nature Pub. Group, 2006- |
| مواضيع طبية MeSH: | Liver* , Organ Preservation*, Cryopreservation/*methods , Cryoprotective Agents/*pharmacology , Liver Transplantation/*methods, Animals ; Rats |
| مستخلص: | The current standard for liver preservation involves cooling of the organ on ice (0-4 °C). Although it is successful for shorter durations, this method of preservation does not allow long-term storage of the liver. The gradual loss of hepatic viability during preservation puts pressure on organ sharing and allocation, may limit the use of suboptimal grafts and necessitates rushed transplantation to achieve desirable post-transplantation outcomes. In an attempt to improve and prolong liver viability during storage, alternative preservation methods are under investigation. For instance, ex vivo machine perfusion systems aim to sustain and even improve viability by supporting hepatic function at warm temperatures, rather than simply slowing down deterioration by cooling. Here we describe a novel subzero preservation technique that combines ex vivo machine perfusion with cryoprotectants to facilitate long-term supercooled preservation. The technique improves the preservation of rat livers to prolong storage times as much as threefold, which is validated by successful long-term recipient survival after orthotopic transplantation. This protocol describes how to load rat livers with cryoprotectants to prevent both intracellular and extracellular ice formation and to protect against hypothermic injury. Cryoprotectants are loaded ex vivo using subnormothermic machine perfusion (SNMP), after which livers can be cooled to -6 °C without freezing and kept viable for up to 96 h. Cooling to a supercooled state is controlled, followed by 3 h of SNMP recovery and orthotopic liver transplantation. |
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| معلومات مُعتمدة: | R00 DK080942 United States DK NIDDK NIH HHS; R01 DK096075 United States DK NIDDK NIH HHS; R01EB008678 United States EB NIBIB NIH HHS; R00DK080942 United States DK NIDDK NIH HHS; R01DK096075 United States DK NIDDK NIH HHS; K08 DK094965 United States DK NIDDK NIH HHS; R01 EB008678 United States EB NIBIB NIH HHS |
| المشرفين على المادة: | 0 (Cryoprotective Agents) |
| تواريخ الأحداث: | Date Created: 20150219 Date Completed: 20151019 Latest Revision: 20220331 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC4494653 |
| DOI: | 10.1038/nprot.2015.011 |
| PMID: | 25692985 |
| قاعدة البيانات: | MEDLINE |
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