دورية أكاديمية
Isolation of chitosan from Ganoderma lucidum mushroom for biomedical applications.
| العنوان: | Isolation of chitosan from Ganoderma lucidum mushroom for biomedical applications. |
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| المؤلفون: | Mesa Ospina N; Biomaterials Research Group, Bioengineering Program, Engineering Faculty, University of Antioquia UdeA, Calle 70 No. 52-21, Medellín, Colombia., Ospina Alvarez SP, Escobar Sierra DM, Rojas Vahos DF, Zapata Ocampo PA, Ossa Orozco CP |
| المصدر: | Journal of materials science. Materials in medicine [J Mater Sci Mater Med] 2015 Mar; Vol. 26 (3), pp. 135. Date of Electronic Publication: 2015 Feb 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: United States NLM ID: 9013087 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4838 (Electronic) Linking ISSN: 09574530 NLM ISO Abbreviation: J Mater Sci Mater Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2008->: Norwell, MA : Springer Original Publication: London : Chapman and Hall, c1990- |
| مواضيع طبية MeSH: | Biocompatible Materials*, Chitosan/*isolation & purification , Reishi/*chemistry, Acetylation ; Animals ; Biomass ; Cell Line ; Chitosan/chemistry ; Mice ; Spectroscopy, Fourier Transform Infrared ; Thermogravimetry ; X-Ray Diffraction |
| مستخلص: | Chitin biopolymer production and its by-product chitosan show great potential. These biomaterials have great applicability in various fields because they are non-toxic, biodegradable, biocompatible, and have antimicrobial effects. The most common source of chitin and chitosan is the crustaceous shell; however, mushrooms are an alternative source for isolating these biopolymers because their cellular wall has a high content of chitin, which may be transformed into chitosan through a deacetylation reaction. The main objective of this research was to obtain chitosan through the deacetylation of chitin isolated from the Ganoderma lucidum basidiomycetes mushroom, which is obtained through biotechnological culture. The material characterization was performed using X-ray diffraction, Fourier transform infrared spectroscopy, thermogravimetric analysis, and an evaluation of cytotoxicity comparing the results obtained with results for commercial chitosan. Protocol results showed that chitosan obtained from this mushroom had a significant similitude with commercial chitosan, yet the one obtained using P2 protocol was the one that rendered the best results: including diffractogram peaks, characteristic infrared analysis bands, and an 80.29 % degree of deacetylation. Cytotoxicity in vitro testing showed that the material was non-toxic; furthermore, it rendered very promising information regarding the evaluation of future applications of this biomaterial in the field of biomedicine. |
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| المشرفين على المادة: | 0 (Biocompatible Materials) 9012-76-4 (Chitosan) |
| تواريخ الأحداث: | Date Created: 20150227 Date Completed: 20160317 Latest Revision: 20181113 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1007/s10856-015-5461-z |
| PMID: | 25716022 |
| قاعدة البيانات: | MEDLINE |
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