دورية أكاديمية
Low-protein diet disrupts the crosstalk between the PKA and PKC signaling pathways in isolated pancreatic islets.
| العنوان: | Low-protein diet disrupts the crosstalk between the PKA and PKC signaling pathways in isolated pancreatic islets. |
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| المؤلفون: | Lippo BR; Department of Pharmaceutical Sciences, Federal University of Pernambuco, 50740-600, Recife, PE, Brazil., Batista TM; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, 13083-865, SP, Brazil., de Rezende LF; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, 13083-865, SP, Brazil., Cappelli AP; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, 13083-865, SP, Brazil., Camargo RL; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, 13083-865, SP, Brazil., Branco RC; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, 13083-865, SP, Brazil., Sampaio HC; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, 13083-865, SP, Brazil., Protzek AO; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, 13083-865, SP, Brazil., Wanderley MI; Department of Physiology and Pharmacology, Federal University of Pernambuco, 50670-901, Recife, PE, Brazil., Arantes VC; Department of Food and Nutrition, Faculty of Nutrition, Federal University of Mato Grosso, 78060-900, Cuiabá, MT, Brazil., Corat MA; Multidisciplinary Center for Biological Research, Unity of Transgenesis, State University of Campinas, 13083-877, SP, Brazil., Carneiro EM; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, 13083-865, SP, Brazil., Udrisar DP; Department of Physiology and Pharmacology, Federal University of Pernambuco, 50670-901, Recife, PE, Brazil., Wanderley AG; Department of Pharmaceutical Sciences, Federal University of Pernambuco, 50740-600, Recife, PE, Brazil; Department of Physiology and Pharmacology, Federal University of Pernambuco, 50670-901, Recife, PE, Brazil., Ferreira F; Department of Physiology and Pharmacology, Federal University of Pernambuco, 50670-901, Recife, PE, Brazil. Electronic address: fabiano.fdino@gmail.com. |
| المصدر: | The Journal of nutritional biochemistry [J Nutr Biochem] 2015 May; Vol. 26 (5), pp. 556-62. Date of Electronic Publication: 2015 Feb 12. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 9010081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4847 (Electronic) Linking ISSN: 09552863 NLM ISO Abbreviation: J Nutr Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <1996->: New York, NY : Elsevier Science Original Publication: Stoneham, MA, USA : Butterworths, c1990- |
| مواضيع طبية MeSH: | Diet, Protein-Restricted* , Signal Transduction*, Cyclic AMP-Dependent Protein Kinases/*metabolism , Islets of Langerhans/*metabolism , Protein Kinase C/*metabolism, Animals ; In Vitro Techniques ; Islets of Langerhans/enzymology ; Male ; Rats ; Rats, Wistar |
| مستخلص: | Protein restriction in the early stages of life can result in several changes in pancreatic function. These alterations include documented reductions in insulin secretion and in cytoplasmic calcium concentration [Ca(2+)]i. However, the mechanisms underlying these changes have not been completely elucidated and may result, in part, from alterations in signaling pathways that potentiate insulin secretion in the presence of glucose. Our findings suggest that protein restriction disrupts the insulin secretory synergism between Cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) and Ca(2+)-dependent protein kinase C (PKC) in isolated islets. Western blot analysis demonstrated reduced levels of both phospho-cAMP response element-binding protein (phospho-CREB) at Ser-133 and substrates phosphorylated by PKCs (Phospho-(Ser) PKC substrate), suggesting that PKA and PKC activity was impaired in islets from rats fed a low-protein diet (LP). cAMP levels and global Ca(2+) entry were also reduced in LP islets. In summary, our findings showed that protein restriction altered the crosstalk between PKA and PKC signaling pathways, resulting in the alteration of secretory synergism in isolated islets. (Copyright © 2015 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Crosstalk; Insulin secretion; Low-protein diet; PKA; PKC |
| المشرفين على المادة: | EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases) EC 2.7.11.13 (Protein Kinase C) |
| تواريخ الأحداث: | Date Created: 20150305 Date Completed: 20160203 Latest Revision: 20150429 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.jnutbio.2014.12.010 |
| PMID: | 25736482 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4847 |
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| DOI: | 10.1016/j.jnutbio.2014.12.010 |