دورية أكاديمية
Characterization of mucosa-associated bacterial communities in abomasal ulcers by pyrosequencing.
| العنوان: | Characterization of mucosa-associated bacterial communities in abomasal ulcers by pyrosequencing. |
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| المؤلفون: | Hund A; University Clinic for Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, 1210 Vienna, Austria., Dzieciol M; Institute for Milk Hygiene, Milk Technology and Food Science, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, 1210 Vienna, Austria., Schmitz-Esser S; Institute for Milk Hygiene, Milk Technology and Food Science, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, 1210 Vienna, Austria; Research Cluster Animal Gut Health, University of Veterinary Medicine Vienna, 1210 Vienna, Austria. Electronic address: stephan.schmitz-esser@vetmeduni.ac.at., Wittek T; University Clinic for Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, 1210 Vienna, Austria. |
| المصدر: | Veterinary microbiology [Vet Microbiol] 2015 May 15; Vol. 177 (1-2), pp. 132-41. Date of Electronic Publication: 2015 Mar 02. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7705469 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2542 (Electronic) Linking ISSN: 03781135 NLM ISO Abbreviation: Vet Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier Scientific Pub. Co. |
| مواضيع طبية MeSH: | Bacteria/*genetics , Cattle Diseases/*microbiology , Gastrointestinal Tract/*microbiology , Microbiota/*genetics , Mucous Membrane/*microbiology , Ulcer/*veterinary, Animals ; Austria ; Base Sequence ; Cattle ; DNA, Bacterial/genetics ; Female ; Male ; Molecular Sequence Data ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA/veterinary ; Ulcer/microbiology |
| مستخلص: | Abomasal ulcers are important pathological alterations of the gastrointestinal tract in cattle and are exceptionally hard to diagnose in vivo. The microbiome of the abomasum in cattle with or without ulcers has hardly been studied to date, and if so, the studies used culture-dependent methods. In the present study, the bacterial communities associated with abomasal ulcers of slaughter cows, bulls, and calves in Austria were described using 16S rRNA gene pyrosequencing. Sequences were clustered into 10,459 operational taxonomic units (OTUs), affiliating to 28 phyla with Proteobacteria, Firmicutes, Bacteroidetes and Tenericutes dominating (96.4% of all reads). The most abundant genera belonged to Helicobacter, Acetobacter, Lactobacillus, and novel Mycoplasma-like phylotypes. Significant differences between the microbial communities of healthy and ulcerated calves compared to cows and bulls could be observed. However, only few statistically significant differences in the abundances of certain OTUs between healthy and ulcerated abomasal mucosa were found. Additionally, near full-length 16S rRNA gene sequences of the most abundant phylotypes were obtained by cloning and Sanger sequencing (n=88). In conclusion, our results allow the first deep insights into the composition of abomasal mucosal bacterial communities in cattle and describe a hitherto unknown high diversity and species richness of abomasal bacteria in cattle. Our results suggest that bacteria may have only limited involvement in the etiology of abomasal ulcers. However, future research will be needed to verify the contribution of bacteria to abomasal ulcer formation as presence or absence of bacteria does not necessarily correlate with etiology of disease. (Copyright © 2015 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 16S rRNA amplicon pyrosequencing; Abomasal ulcers; Abomasum; Bacterial microbiome; Cattle |
| المشرفين على المادة: | 0 (DNA, Bacterial) 0 (RNA, Ribosomal, 16S) |
| تواريخ الأحداث: | Date Created: 20150316 Date Completed: 20160108 Latest Revision: 20181202 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.vetmic.2015.02.023 |
| PMID: | 25770891 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2542 |
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| DOI: | 10.1016/j.vetmic.2015.02.023 |