دورية أكاديمية
Resveratrol Regulates the Quiescence-Like Induction of Activated Stellate Cells by Modulating the PPARγ/SIRT1 Ratio.
| العنوان: | Resveratrol Regulates the Quiescence-Like Induction of Activated Stellate Cells by Modulating the PPARγ/SIRT1 Ratio. |
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| المؤلفون: | de Souza IC; Departamento de Morfologia, IB, Universidade Federal de Pelotas (UFPel), av. Duque de Caxias, 250, CEP 96 030 000, Pelotas, RS, Brazil.; Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), rua Ramiro Barcelos, 2600-Anexo I, CEP 90035-003, Porto Alegre, RS, Brazil., Martins LA; Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), rua Ramiro Barcelos, 2600-Anexo I, CEP 90035-003, Porto Alegre, RS, Brazil., de Vasconcelos M; Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), rua Ramiro Barcelos, 2600-Anexo I, CEP 90035-003, Porto Alegre, RS, Brazil., de Oliveira CM; Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), rua Ramiro Barcelos, 2600-Anexo I, CEP 90035-003, Porto Alegre, RS, Brazil., Barbé-Tuana F; Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), rua Ramiro Barcelos, 2600-Anexo I, CEP 90035-003, Porto Alegre, RS, Brazil., Andrade CB; Departamento de Química, ICET, Universidade Federal do Mato Grosso (UFMT), Cuiabá, MT, Brazil., Pettenuzzo LF; Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), rua Ramiro Barcelos, 2600-Anexo I, CEP 90035-003, Porto Alegre, RS, Brazil., Borojevic R; Departamento de Histologia e Embriologia, ICB, PABCAM, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil., Margis R; Departamento de Biofísica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil., Guaragna R; Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), rua Ramiro Barcelos, 2600-Anexo I, CEP 90035-003, Porto Alegre, RS, Brazil., Guma FC; Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul (UFRGS), rua Ramiro Barcelos, 2600-Anexo I, CEP 90035-003, Porto Alegre, RS, Brazil. |
| المصدر: | Journal of cellular biochemistry [J Cell Biochem] 2015 Oct; Vol. 116 (10), pp. 2304-12. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8205768 Publication Model: Print Cited Medium: Internet ISSN: 1097-4644 (Electronic) Linking ISSN: 07302312 NLM ISO Abbreviation: J Cell Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2004>- : Hoboken, NJ : Wiley-Liss Original Publication: New York : Liss, c1982- |
| مواضيع طبية MeSH: | Fibrosis/*genetics , Lipid Droplets/*metabolism , PPAR gamma/*biosynthesis , Sirtuin 1/*biosynthesis, Animals ; Cell Proliferation ; Fibrosis/pathology ; Gene Expression Regulation/drug effects ; Hepatic Stellate Cells/metabolism ; Humans ; Kupffer Cells/metabolism ; Lipogenesis/drug effects ; Liver/metabolism ; Mice ; Myoblasts/metabolism ; PPAR gamma/metabolism ; RNA, Messenger/biosynthesis ; Resveratrol ; Sirtuin 1/metabolism ; Stilbenes/administration & dosage ; Vitamin A/metabolism |
| مستخلص: | The activation of hepatic stellate cell (HSC), from a quiescent cell featuring cytoplasmic lipid droplets to a proliferative myofibroblast, plays an important role in liver fibrosis development. The GRX line is an activated HSC model that can be induced by all-trans-retinol to accumulate lipid droplets. Resveratrol is known for activating Sirtuin1 (SIRT1), a NAD(+)-dependent deacetylase that suppresses the activity of peroxisome proliferator-activated receptor gamma (PPARγ), an important adipogenic transcription factor involved in the quiescence maintenance of HSC. We evaluated the effects of 0.1 μM of resveratrol in retinol-induced GRX quiescence by investigating the interference of SIRT1 and PPARγ on cell lipogenesis. GRX lipid accumulation was evaluated through Oil-red O staining, triacylglycerides quantification, and [(14)C] acetate incorporation into lipids. mRNA expression and protein content of SIRT1 and PPARγ were measured by RT-PCR and immunoblotting, respectively. Resveratrol-mediated SIRT1 stimuli did not induce lipogenesis and reduced the retinol-mediated fat-storing capacity in GRX. In order to support our results, we established a cell culture model of transgenic super expression of PPARγ in GRX cells (GRXPγ). Resveratrol reduced lipid droplets accumulation in GRXPγ cells. These results suggest that the PPARγ/SIRT1 ratio plays an important role in the fate of HSC. Thus, whenever the PPARγ activity is greater than SIRT1 activity the lipogenesis is enabled. (© 2015 Wiley Periodicals, Inc.) |
| فهرسة مساهمة: | Keywords: GRX; HEPATIC STELLATE CELLS; LIPID ACCUMULATION; PPARγ; RESVERATROL; SIRT1 |
| المشرفين على المادة: | 0 (PPAR gamma) 0 (RNA, Messenger) 0 (Stilbenes) 11103-57-4 (Vitamin A) EC 3.5.1.- (Sirt1 protein, mouse) EC 3.5.1.- (Sirtuin 1) Q369O8926L (Resveratrol) |
| تواريخ الأحداث: | Date Created: 20150403 Date Completed: 20160531 Latest Revision: 20181202 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1002/jcb.25181 |
| PMID: | 25833683 |
| قاعدة البيانات: | MEDLINE |
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