دورية أكاديمية
أعرض في EDS

Ubiquitous L1 mosaicism in hippocampal neurons.

التفاصيل البيبلوغرافية
العنوان: Ubiquitous L1 mosaicism in hippocampal neurons.
المؤلفون: Upton KR; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia., Gerhardt DJ; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia., Jesuadian JS; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia., Richardson SR; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia., Sánchez-Luque FJ; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia., Bodea GO; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia., Ewing AD; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia., Salvador-Palomeque C; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia., van der Knaap MS; Department of Child Neurology, Neuroscience Campus Amsterdam, VU University Medical Center, 1081 HV Amsterdam, The Netherlands., Brennan PM; Edinburgh Cancer Research Centre, Western General Hospital, Edinburgh, EH4 2XR, UK., Vanderver A; Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC 20010, USA., Faulkner GJ; Mater Research Institute - University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia; Queensland Brain Institute, University of Queensland, Brisbane QLD 4072, Australia. Electronic address: faulknergj@gmail.com.
المصدر: Cell [Cell] 2015 Apr 09; Vol. 161 (2), pp. 228-39.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 0413066 Publication Model: Print Cited Medium: Internet ISSN: 1097-4172 (Electronic) Linking ISSN: 00928674 NLM ISO Abbreviation: Cell Subsets: MEDLINE
أسماء مطبوعة: Publication: Cambridge, Ma : Cell Press
Original Publication: Cambridge, MIT Press.
مواضيع طبية MeSH: Long Interspersed Nucleotide Elements* , Mosaicism*, Hippocampus/*cytology , Neurons/*cytology, Genetic Variation ; Humans ; Neurogenesis ; Polymerase Chain Reaction ; Tissue Banks
مستخلص: Somatic LINE-1 (L1) retrotransposition during neurogenesis is a potential source of genotypic variation among neurons. As a neurogenic niche, the hippocampus supports pronounced L1 activity. However, the basal parameters and biological impact of L1-driven mosaicism remain unclear. Here, we performed single-cell retrotransposon capture sequencing (RC-seq) on individual human hippocampal neurons and glia, as well as cortical neurons. An estimated 13.7 somatic L1 insertions occurred per hippocampal neuron and carried the sequence hallmarks of target-primed reverse transcription. Notably, hippocampal neuron L1 insertions were specifically enriched in transcribed neuronal stem cell enhancers and hippocampus genes, increasing their probability of functional relevance. In addition, bias against intronic L1 insertions sense oriented relative to their host gene was observed, perhaps indicating moderate selection against this configuration in vivo. These experiments demonstrate pervasive L1 mosaicism at genomic loci expressed in hippocampal neurons.
(Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: 090386/Z/09/Z United Kingdom Wellcome Trust
تواريخ الأحداث: Date Created: 20150411 Date Completed: 20150702 Latest Revision: 20220408
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC4398972
DOI: 10.1016/j.cell.2015.03.026
PMID: 25860606
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-4172
DOI:10.1016/j.cell.2015.03.026