دورية أكاديمية
Characterization of the microbiota in the guts of Triatoma brasiliensis and Triatoma pseudomaculata infected by Trypanosoma cruzi in natural conditions using culture independent methods.
العنوان: | Characterization of the microbiota in the guts of Triatoma brasiliensis and Triatoma pseudomaculata infected by Trypanosoma cruzi in natural conditions using culture independent methods. |
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المؤلفون: | Gumiel M; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, RJ, Brazil. mgumiel@ioc.fiocruz.br., da Mota FF; Laboratório de Biologia Computacional e Sistemas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, RJ, Brazil. fabio@ioc.fiocruz.br.; Departamento de Entomologia Molecular, Instituto Nacional de Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil. fabio@ioc.fiocruz.br., Rizzo Vde S; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, RJ, Brazil. vanessa.rizzo7@gmail.com., Sarquis O; Laboratório de Ecoepidemiologia da Doença de Chagas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, RJ, Brazil. otiliasarquis@ioc.fiocruz.br., de Castro DP; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, RJ, Brazil. didan.castro@gmail.com.; Departamento de Entomologia Molecular, Instituto Nacional de Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil. didan.castro@gmail.com., Lima MM; Laboratório de Ecoepidemiologia da Doença de Chagas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, RJ, Brazil. mmlima@ioc.fiocruz.br., Garcia Ede S; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, RJ, Brazil. garciaeloi@gmail.com.; Departamento de Entomologia Molecular, Instituto Nacional de Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil. garciaeloi@gmail.com., Carels N; Laboratório de Modelagem de Sistemas Biológicos, National Institute for Science and Technology on Innovation in Neglected Diseases (INCT-IDN), Centro de Desenvolvimento Tecnológico em Saúde (CDTS), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, RJ, Brazil. nicolas.carels@cdts.fiocruz.br., Azambuja P; Laboratório de Bioquímica e Fisiologia de Insetos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro, RJ, Brazil. azambuja.p@gmail.com.; Departamento de Entomologia Molecular, Instituto Nacional de Entomologia Molecular (INCT-EM), Rio de Janeiro, RJ, Brazil. azambuja.p@gmail.com. |
المصدر: | Parasites & vectors [Parasit Vectors] 2015 Apr 24; Vol. 8, pp. 245. Date of Electronic Publication: 2015 Apr 24. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : BioMed Central |
مواضيع طبية MeSH: | Triatoma/*microbiology , Triatoma/*parasitology , Trypanosoma cruzi/*isolation & purification, Animals ; Bacteria/classification ; Bacteria/genetics ; DNA, Bacterial ; Denaturing Gradient Gel Electrophoresis ; Electron Transport Complex IV/genetics ; Female ; Gastrointestinal Tract/microbiology ; Gene Expression Regulation, Bacterial ; Nymph/microbiology ; Phylogeny ; Polymerase Chain Reaction ; RNA, Bacterial/genetics ; Triatoma/classification ; Triatoma/genetics |
مستخلص: | Background: Chagas disease is caused by Trypanosoma cruzi, which is transmitted by triatomine vectors. The northeastern region of Brazil is endemic for Chagas disease and has the largest diversity of triatomine species. T. cruzi development in its triatomine vector depends on diverse factors, including the composition of bacterial gut microbiota. Methods: We characterized the triatomines captured in the municipality of Russas (Ceará) by sequencing the cytochrome c oxidase subunit I (COI) gene. The composition of the bacterial community in the gut of peridomestic Triatoma brasiliensis and Triatoma pseudomaculata was investigated using culture independent methods based on the amplification of the 16S rRNA gene by polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), DNA fragment cloning, Sanger sequencing and 454 pyrosequencing. Additionally, we identified TcI and TcII types of T. cruzi by sequencing amplicons from the gut metagenomic DNA with primers for the mini-exon gene. Results: Triatomines collected in the peridomestic ecotopes were diagnosed as T. pseudomaculata and T. brasiliensis by comparing their COI sequence with GenBank. The rate of infection by T. cruzi in adult triatomines reached 80% for T. pseudomaculata and 90% for T. brasiliensis. According to the DNA sequences from the DGGE bands, the triatomine gut microbiota was primarily composed of Proteobacteria and Actinobacteria. However, Firmicutes and Bacteroidetes were also detected, although in much lower proportions. Serratia was the main genus, as it was encountered in all samples analyzed by DGGE and 454 pyrosequencing. Members of Corynebacterinae, a suborder of the Actinomycetales, formed the next most important group. The cloning and sequencing of full-length 16S rRNA genes confirmed the presence of Serratia marcescens, Dietzia sp., Gordonia terrae, Corynebacterium stationis and Corynebacterium glutamicum. Conclusions: The study of the bacterial microbiota in the triatomine gut has gained increased attention because of the possible role it may play in the epidemiology of Chagas disease by competing with T. cruzi. Culture independent methods have shown that the bacterial composition of the microbiota in the guts of peridomestic triatomines is made up by only few bacterial species. |
References: | Trans R Soc Trop Med Hyg. 2001 Jan-Feb;95(1):97-9. (PMID: 11280078) Int J Parasitol. 2001 May 1;31(5-6):621-7. (PMID: 11334952) Trans R Soc Trop Med Hyg. 2012 Mar;106(3):143-9. (PMID: 22136953) PLoS One. 2013;8(4):e61218. (PMID: 23613815) Am J Trop Med Hyg. 2012 Apr;86(4):677-82. (PMID: 22492153) J Bacteriol. 1996 Oct;178(19):5636-43. (PMID: 8824607) Cad Saude Publica. 2000;16 Suppl 2:43-59. (PMID: 11119319) Genetica. 2004 Jul;121(3):229-40. (PMID: 15521421) Parasitol Today. 1998 Mar;14(3):119-24. (PMID: 17040719) J Med Microbiol. 2007 Dec;56(Pt 12):1687-8. (PMID: 18033840) Trends Parasitol. 2010 Oct;26(10):499-505. (PMID: 20801082) Exp Parasitol. 2007 Oct;117(2):201-7. (PMID: 17570364) Mol Mar Biol Biotechnol. 1994 Oct;3(5):294-9. (PMID: 7881515) Am J Trop Med Hyg. 1999 Mar;60(3):377-86. (PMID: 10466963) Antonie Van Leeuwenhoek. 1986;52(2):129-40. (PMID: 3729373) Exp Parasitol. 2008 May;119(1):94-8. (PMID: 18331732) PLoS One. 2012;7(5):e36591. (PMID: 22574189) Mol Ecol. 2012 Dec;21(24):6134-51. (PMID: 23017151) FEMS Microbiol Ecol. 2001 Mar;35(1):27-36. (PMID: 11248387) Dev Comp Immunol. 2014 Jan;42(1):111-23. (PMID: 23764256) Mol Biol Evol. 2011 Oct;28(10):2731-9. (PMID: 21546353) Infect Genet Evol. 2014 Apr;23:74-9. (PMID: 24508245) Mem Inst Oswaldo Cruz. 2004 May;99(3):263-70. (PMID: 15273797) Parasitol Res. 2013 Apr;112(4):1543-50. (PMID: 23371496) Bioinformatics. 2011 Aug 15;27(16):2194-200. (PMID: 21700674) Res Microbiol. 1993 Oct;144(8):665-72. (PMID: 8140285) Curr Mol Med. 2004 Sep;4(6):549-62. (PMID: 15357207) Parasitol Res. 2012 Apr;110(4):1481-5. (PMID: 21979785) Cad Saude Publica. 2000;16 Suppl 2:61-7. (PMID: 11119320) Parasit Vectors. 2014;7:232. (PMID: 24885969) Exp Parasitol. 2002 Jan;100(1):17-27. (PMID: 11971650) Mem Inst Oswaldo Cruz. 2009 Nov;104(7):1051-4. (PMID: 20027478) Acta Trop. 2009 May-Jun;110(2-3):137-47. (PMID: 18992212) Mol Ecol. 2012 Oct;21(20):5124-37. (PMID: 22978555) Infect Genet Evol. 2009 Jan;9(1):54-61. (PMID: 18992369) Mem Inst Oswaldo Cruz. 2003 Jun;98(4):443-9. (PMID: 12937751) Parasitology. 2009 Oct;136(12):1509-28. (PMID: 19691868) Appl Environ Microbiol. 2007 Aug;73(16):5261-7. (PMID: 17586664) PLoS Biol. 2008 Nov 18;6(11):e280. (PMID: 19018661) Appl Environ Microbiol. 2008 Feb;74(3):889-93. (PMID: 18083874) Am J Trop Med Hyg. 2010 Sep;83(3):519-22. (PMID: 20810814) Genome Announc. 2014 Oct 09;2(5):null. (PMID: 25301659) J Trop Med. 2012;2012:705326. (PMID: 22523500) Rev Soc Bras Med Trop. 2012 Feb;45(1):60-5. (PMID: 22370830) J Mol Biol. 1990 Oct 5;215(3):403-10. (PMID: 2231712) Appl Environ Microbiol. 2012 Apr;78(8):2830-40. (PMID: 22307297) Am J Trop Med Hyg. 1951 May;31(3):381-2. (PMID: 14829752) Mol Ecol. 2005 Aug;14(9):2637-43. (PMID: 16029466) Exp Parasitol. 2004 May-Jun;107(1-2):89-96. (PMID: 15208042) Nucleic Acids Res. 2009 Jan;37(Database issue):D141-5. (PMID: 19004872) Parasit Vectors. 2011;4:66. (PMID: 21548954) Exp Parasitol. 2007 Feb;115(2):200-4. (PMID: 16989812) PLoS Negl Trop Dis. 2012;6(5):e1631. (PMID: 22563511) PLoS One. 2013;8(12):e81663. (PMID: 24324714) J Gen Microbiol. 1978 Dec;109(2):215-23. (PMID: 745002) PLoS Comput Biol. 2007 Mar 30;3(3):e56. (PMID: 17397253) Am J Trop Med Hyg. 2014 Jun;90(6):1059-62. (PMID: 24710611) Appl Environ Microbiol. 2009 Jan;75(2):547-50. (PMID: 19028913) Infect Genet Evol. 2012 Mar;12(2):240-53. (PMID: 22226704) PLoS One. 2008;3(7):e2836. (PMID: 18665274) Int J Syst Bacteriol. 1997 Oct;47(4):1140-4. (PMID: 9336921) J Microbiol Biotechnol. 2008 May;18(5):805-14. (PMID: 18633275) Biochem Genet. 2005 Oct;43(9-10):519-30. (PMID: 16341768) Curr Microbiol. 2002 Feb;44(2):120-4. (PMID: 11815856) Am J Trop Med Hyg. 2013 Oct;89(4):766-74. (PMID: 24002487) Appl Environ Microbiol. 2004 Aug;70(8):4800-6. (PMID: 15294817) J Biomed Biotechnol. 2010;2010:890672. (PMID: 20396398) Parasit Vectors. 2014;7:94. (PMID: 24593987) FEMS Microbiol Rev. 2013 Sep;37(5):699-735. (PMID: 23692388) Antonie Van Leeuwenhoek. 2010 Jan;97(1):69-77. (PMID: 19876756) Mem Inst Oswaldo Cruz. 2014 Nov;109(7):887-98. (PMID: 25410992) Mem Inst Oswaldo Cruz. 2006 Mar;101(2):143-7. (PMID: 16830706) Int J Parasitol. 2000 Jan;30(1):35-44. (PMID: 10675742) Trends Parasitol. 2005 Dec;21(12):568-72. (PMID: 16226491) PLoS One. 2012;7(8):e42531. (PMID: 22880020) FEMS Microbiol Lett. 1998 Jan 1;158(1):1-8. (PMID: 9453150) Cad Saude Publica. 2014 Apr;30(4):785-93. (PMID: 24896053) J Bacteriol. 1994 Jan;176(1):1-6. (PMID: 8282683) Emerg Infect Dis. 1998 Oct-Dec;4(4):581-91. (PMID: 9866734) Bioinformatics. 2007 Nov 1;23(21):2947-8. (PMID: 17846036) Am J Trop Med Hyg. 2004 Jun;70(6):597-603. (PMID: 15210998) J Med Entomol. 2001 Sep;38(5):675-83. (PMID: 11580040) Mem Inst Oswaldo Cruz. 2010 May;105(3):348-52. (PMID: 20512253) Int J Syst Evol Microbiol. 2010 Apr;60(Pt 4):874-9. (PMID: 19661509) |
المشرفين على المادة: | 0 (DNA, Bacterial) 0 (RNA, Bacterial) EC 1.9.3.1 (Electron Transport Complex IV) |
تواريخ الأحداث: | Date Created: 20150424 Date Completed: 20160125 Latest Revision: 20181113 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC4429471 |
DOI: | 10.1186/s13071-015-0836-z |
PMID: | 25903360 |
قاعدة البيانات: | MEDLINE |
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