دورية أكاديمية
A versatile clearing agent for multi-modal brain imaging.
| العنوان: | A versatile clearing agent for multi-modal brain imaging. |
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| المؤلفون: | Costantini I; European Laboratory for Non-linear Spectroscopy, University of Florence, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy., Ghobril JP; Laboratory of Neural Microcircuitry, Brain Mind Institute, EPFL, Station 15, CH-1015 Lausanne, Switzerland., Di Giovanna AP; European Laboratory for Non-linear Spectroscopy, University of Florence, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy., Allegra Mascaro AL; European Laboratory for Non-linear Spectroscopy, University of Florence, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy., Silvestri L; European Laboratory for Non-linear Spectroscopy, University of Florence, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy., Müllenbroich MC; European Laboratory for Non-linear Spectroscopy, University of Florence, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy., Onofri L; European Laboratory for Non-linear Spectroscopy, University of Florence, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy., Conti V; Pediatric Neurology and Neurogenetics Unit and Laboratories, Department of Neuroscience, Pharmacology and Child Health, A. Meyer Children's Hospital - University of Florence, Viale Pieraccini 24, 50139 Florence, Italy., Vanzi F; 1] European Laboratory for Non-linear Spectroscopy, University of Florence, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy [2] Department of Biology, University of Florence, Via Romana 17, 50125 Florence, Italy., Sacconi L; 1] National Institute of Optics, National Research Council, Largo Fermi 6, 50125 Florence, Italy [2] European Laboratory for Non-linear Spectroscopy, University of Florence, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy., Guerrini R; Pediatric Neurology and Neurogenetics Unit and Laboratories, Department of Neuroscience, Pharmacology and Child Health, A. Meyer Children's Hospital - University of Florence, Viale Pieraccini 24, 50139 Florence, Italy., Markram H; Laboratory of Neural Microcircuitry, Brain Mind Institute, EPFL, Station 15, CH-1015 Lausanne, Switzerland., Iannello G; Department of Engineering, University Campus Bio-Medico of Rome, Via Alvaro del Portillo 21, 00128 Roma, Italy., Pavone FS; 1] European Laboratory for Non-linear Spectroscopy, University of Florence, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy [2] National Institute of Optics, National Research Council, Largo Fermi 6, 50125 Florence, Italy [3] Department of Physics and Astronomy, University of Florence, Via Sansone 1, 50019 Sesto Fiorentino, Italy. |
| المصدر: | Scientific reports [Sci Rep] 2015 May 07; Vol. 5, pp. 9808. Date of Electronic Publication: 2015 May 07. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
| مواضيع طبية MeSH: | Brain* , Contrast Media*, Neuroimaging/*methods, Animals ; Humans ; Immunohistochemistry/methods ; Mice ; Tomography/methods |
| مستخلص: | Extensive mapping of neuronal connections in the central nervous system requires high-throughput µm-scale imaging of large volumes. In recent years, different approaches have been developed to overcome the limitations due to tissue light scattering. These methods are generally developed to improve the performance of a specific imaging modality, thus limiting comprehensive neuroanatomical exploration by multi-modal optical techniques. Here, we introduce a versatile brain clearing agent (2,2'-thiodiethanol; TDE) suitable for various applications and imaging techniques. TDE is cost-efficient, water-soluble and low-viscous and, more importantly, it preserves fluorescence, is compatible with immunostaining and does not cause deformations at sub-cellular level. We demonstrate the effectiveness of this method in different applications: in fixed samples by imaging a whole mouse hippocampus with serial two-photon tomography; in combination with CLARITY by reconstructing an entire mouse brain with light sheet microscopy and in translational research by imaging immunostained human dysplastic brain tissue. |
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| المشرفين على المادة: | 0 (Contrast Media) |
| تواريخ الأحداث: | Date Created: 20150508 Date Completed: 20160304 Latest Revision: 20220330 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC4423470 |
| DOI: | 10.1038/srep09808 |
| PMID: | 25950610 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2045-2322 |
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| DOI: | 10.1038/srep09808 |