دورية أكاديمية
Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle.
| العنوان: | Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle. |
|---|---|
| المؤلفون: | Zhang T; Department of Internal Medicine-Gerontology, Wake Forest School of Medicine, Winston-Salem, NC 27157, USA., Taylor J; Department of Internal Medicine-Gerontology, Wake Forest School of Medicine, Winston-Salem, NC 27157, USA., Jiang Y; Department of Internal Medicine-Gerontology, Wake Forest School of Medicine, Winston-Salem, NC 27157, USA., Pereyra AS; Department of Histology, National University of La Plata, 1900 La Plata, Argentina., Messi ML; Department of Internal Medicine-Gerontology, Wake Forest School of Medicine, Winston-Salem, NC 27157, USA., Wang ZM; Department of Internal Medicine-Gerontology, Wake Forest School of Medicine, Winston-Salem, NC 27157, USA., Hereñú C; Department of Histology, National University of La Plata, 1900 La Plata, Argentina., Delbono O; Department of Internal Medicine-Gerontology, Wake Forest School of Medicine, Winston-Salem, NC 27157, USA; Neuroscience Program, Wake Forest School of Medicine, Winston-Salem, NC 27157, USA. Electronic address: odelbono@wakehealth.edu. |
| المصدر: | Experimental cell research [Exp Cell Res] 2015 Aug 15; Vol. 336 (2), pp. 276-86. Date of Electronic Publication: 2015 May 15. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0373226 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2422 (Electronic) Linking ISSN: 00144827 NLM ISO Abbreviation: Exp Cell Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando Fl : Academic Press Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Calcium Channels/*metabolism , Calcium Channels, L-Type/*metabolism , Excitation Contraction Coupling/*physiology , Muscle Fibers, Skeletal/*metabolism , Troponin/*metabolism, Active Transport, Cell Nucleus ; Animals ; Calcium/metabolism ; Cell Differentiation ; Cell Line ; Cell Nucleus/metabolism ; Gene Library ; Mice ; Mice, Inbred C57BL ; Muscle Contraction/physiology ; Muscle Fibers, Skeletal/cytology ; Transcription, Genetic/genetics |
| مستخلص: | The voltage-gated calcium channel (Cav) β1a subunit (Cavβ1a) plays an important role in excitation-contraction coupling (ECC), a process in the myoplasm that leads to muscle-force generation. Recently, we discovered that the Cavβ1a subunit travels to the nucleus of skeletal muscle cells where it helps to regulate gene transcription. To determine how it travels to the nucleus, we performed a yeast two-hybrid screening of the mouse fast skeletal muscle cDNA library and identified an interaction with troponin T3 (TnT3), which we subsequently confirmed by co-immunoprecipitation and co-localization assays in mouse skeletal muscle in vivo and in cultured C2C12 muscle cells. Interacting domains were mapped to the leucine zipper domain in TnT3 COOH-terminus (160-244 aa) and Cavβ1a NH2-terminus (1-99 aa), respectively. The double fluorescence assay in C2C12 cells co-expressing TnT3/DsRed and Cavβ1a/YFP shows that TnT3 facilitates Cavβ1a nuclear recruitment, suggesting that the two proteins play a heretofore unknown role during early muscle differentiation in addition to their classical role in ECC regulation. (Copyright © 2015 Elsevier Inc. All rights reserved.) |
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| معلومات مُعتمدة: | R01 AG013934 United States AG NIA NIH HHS; R01 AG015820 United States AG NIA NIH HHS; R01AG13934 United States AG NIA NIH HHS; R01AG15820 United States AG NIA NIH HHS |
| فهرسة مساهمة: | Keywords: Ca(v)β(1a); Nuclear localization; Skeletal muscle; Troponin T3 |
| المشرفين على المادة: | 0 (Cacnb1 protein, mouse) 0 (Calcium Channels) 0 (Calcium Channels, L-Type) 0 (Troponin) 0 (troponin T3, skeletal, fast, mouse) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 20150519 Date Completed: 20151027 Latest Revision: 20181113 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC4522384 |
| DOI: | 10.1016/j.yexcr.2015.05.005 |
| PMID: | 25981458 |
| قاعدة البيانات: | MEDLINE |
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