دورية أكاديمية
أعرض في EDS

Vitamin D Binding Protein Isoforms and Apolipoprotein E in Cerebrospinal Fluid as Prognostic Biomarkers of Multiple Sclerosis.

التفاصيل البيبلوغرافية
العنوان: Vitamin D Binding Protein Isoforms and Apolipoprotein E in Cerebrospinal Fluid as Prognostic Biomarkers of Multiple Sclerosis.
المؤلفون: Perga S; Neurology Unit 2 & Regional Referral Multiple Sclerosis Centre (CReSM), San Luigi University Hospital, Orbassano, Italy; Neuroscience Institute Cavalieri Ottolenghi (NICO), c/o San Luigi University Hospital, Orbassano, Italy., Giuliano Albo A; ABLE Biosciences, Bioindustry Park Silvano Fumero SpA, Colleretto Giacosa, Italy; LIMA, Bioindustry Park Silvano Fumero SpA, Colleretto Giacosa, Italy., Lis K; LIMA, Bioindustry Park Silvano Fumero SpA, Colleretto Giacosa, Italy., Minari N; LIMA, Bioindustry Park Silvano Fumero SpA, Colleretto Giacosa, Italy., Falvo S; ABLE Biosciences, Bioindustry Park Silvano Fumero SpA, Colleretto Giacosa, Italy; LIMA, Bioindustry Park Silvano Fumero SpA, Colleretto Giacosa, Italy., Marnetto F; Neurology Unit 2 & Regional Referral Multiple Sclerosis Centre (CReSM), San Luigi University Hospital, Orbassano, Italy; Neuroscience Institute Cavalieri Ottolenghi (NICO), c/o San Luigi University Hospital, Orbassano, Italy., Caldano M; Neurology Unit 2 & Regional Referral Multiple Sclerosis Centre (CReSM), San Luigi University Hospital, Orbassano, Italy; Neuroscience Institute Cavalieri Ottolenghi (NICO), c/o San Luigi University Hospital, Orbassano, Italy., Reviglione R; ABLE Biosciences, Bioindustry Park Silvano Fumero SpA, Colleretto Giacosa, Italy; LIMA, Bioindustry Park Silvano Fumero SpA, Colleretto Giacosa, Italy., Berchialla P; Department of Clinical and Biological Sciences, University of Turin, Turin, Italy., Capobianco MA; Neurology Unit 2 & Regional Referral Multiple Sclerosis Centre (CReSM), San Luigi University Hospital, Orbassano, Italy., Malentacchi M; Neurology Unit 2 & Regional Referral Multiple Sclerosis Centre (CReSM), San Luigi University Hospital, Orbassano, Italy., Corpillo D; ABLE Biosciences, Bioindustry Park Silvano Fumero SpA, Colleretto Giacosa, Italy; LIMA, Bioindustry Park Silvano Fumero SpA, Colleretto Giacosa, Italy., Bertolotto A; Neurology Unit 2 & Regional Referral Multiple Sclerosis Centre (CReSM), San Luigi University Hospital, Orbassano, Italy; Neuroscience Institute Cavalieri Ottolenghi (NICO), c/o San Luigi University Hospital, Orbassano, Italy.
المصدر: PloS one [PLoS One] 2015 Jun 05; Vol. 10 (6), pp. e0129291. Date of Electronic Publication: 2015 Jun 05 (Print Publication: 2015).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Apolipoproteins E/*cerebrospinal fluid , Biomarkers/*cerebrospinal fluid , Multiple Sclerosis/*cerebrospinal fluid , Vitamin D-Binding Protein/*cerebrospinal fluid, Adolescent ; Adult ; Blotting, Western ; Cluster Analysis ; Electrophoresis, Gel, Two-Dimensional ; Female ; Humans ; Mass Spectrometry ; Middle Aged ; Multiple Sclerosis/classification ; Multiple Sclerosis/diagnosis ; Prognosis ; Protein Isoforms/cerebrospinal fluid ; Proteome/classification ; Proteome/metabolism ; Proteomics/methods ; Young Adult
مستخلص: Background: Multiple sclerosis (MS) is a multifactorial autoimmune disease of the central nervous system with a heterogeneous and unpredictable course. To date there are no prognostic biomarkers even if they would be extremely useful for early patient intervention with personalized therapies. In this context, the analysis of inter-individual differences in cerebrospinal fluid (CSF) proteome may lead to the discovery of biological markers that are able to distinguish the various clinical forms at diagnosis.
Methods: To this aim, a two dimensional electrophoresis (2-DE) study was carried out on individual CSF samples from 24 untreated women who underwent lumbar puncture (LP) for suspected MS. The patients were clinically monitored for 5 years and then classified according to the degree of disease aggressiveness and the disease-modifying therapies prescribed during follow up.
Results: The hierarchical cluster analysis of 2-DE dataset revealed three protein spots which were identified by means of mass spectrometry as Apolipoprotein E (ApoE) and two isoforms of vitamin D binding protein (DBP). These three protein spots enabled us to subdivide the patients into subgroups correlated with clinical classification (MS aggressive forms identification: 80%). In particular, we observed an opposite trend of values for the two protein spots corresponding to different DBP isoforms suggesting a role of a post-translational modification rather than the total protein content in patient categorization.
Conclusions: These findings proved to be very interesting and innovative and may be developed as new candidate prognostic biomarkers of MS aggressiveness, if confirmed.
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المشرفين على المادة: 0 (Apolipoproteins E)
0 (Biomarkers)
0 (Protein Isoforms)
0 (Proteome)
0 (Vitamin D-Binding Protein)
تواريخ الأحداث: Date Created: 20150606 Date Completed: 20160401 Latest Revision: 20240326
رمز التحديث: 20240326
مُعرف محوري في PubMed: PMC4457896
DOI: 10.1371/journal.pone.0129291
PMID: 26046356
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0129291