دورية أكاديمية
[Expression Changes of Matrix Reconstitution-related Genes in Bone Marrow during 5-Fluorouracil-Induced Hemopoietic Injury and Their Significance].
| العنوان: | [Expression Changes of Matrix Reconstitution-related Genes in Bone Marrow during 5-Fluorouracil-Induced Hemopoietic Injury and Their Significance]. |
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| المؤلفون: | Zhao L; Department of Anatomy, Qingdao University Medical College, Qingdao 266071, Shandong Province, China., Wu K; Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China., Ning JL; Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China., Wang T; Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China., Shen Y; Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China., Hu YB; Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China., Shao LB; Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China., Li YY; Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China., Wang YQ; Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215007, Jiangsu Province, China., Shen RW; Department of Anatomy, Qingdao University Medical College, Qingdao 266071, Shandong Province, China. E-mail :shenruowu@aliyun.com. |
| المصدر: | Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2015 Jun; Vol. 23 (3), pp. 848-51. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhongguo shi yan xue za zhi she Country of Publication: China NLM ID: 101084424 Publication Model: Print Cited Medium: Print ISSN: 1009-2137 (Print) Linking ISSN: 10092137 NLM ISO Abbreviation: Zhongguo Shi Yan Xue Ye Xue Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Beijing : Zhongguo shi yan xue za zhi she, |
| مواضيع طبية MeSH: | Bone Marrow* , Bone Marrow Cells* , Gene Expression Regulation*, Blood Cells/*pathology, Animals ; Blood Cell Count ; Extracellular Matrix ; Fluorouracil ; Gene Expression ; Mice ; Mice, Inbred C57BL ; Real-Time Polymerase Chain Reaction |
| مستخلص: | Objective: To explore the expression change of the genes related with matrix reconstitution during the injury and reconstitution of murine bone marrow following treatment with 5-fluorouracil (5-FU). Methods: C57BL/6 mice received intraperitoneal injection of 5-FU (200 mg/kg), and peripheral blood cell counts were monitored at 3, 6, 9, 15, 21, 27 days after treatment. Bone marrow cells were harvested at these times for total RNA extraction using TRIzol. Reverse transcriptions in combination with real-time PCR were performed for detecting expression of genes related with matrix reconstitution, including ECM-1, MMP-2, MMP-3, MMP-13 and TIMP-1. Results: After injection of 5-FU, the numbers of three line cells in peripheral blood (i.e. RBC, WBC and platelets) decreased and then recovered with differential dynamics. Similarly, RT-qPCR revealed that all the 5 detected gene expressions were significantly up-regulated during the injury. The mRNA expression of MMP-2 reached to peak at day 3 while the other genes reached to peak at day 6. MMP-3 has a low expression when compared with others, but its expression increased significantly after injury. Conclusion: In 5-FU induced hematopoietic injury and reconstitution model, matrix reconstitution-related genes may play an important role in hematopoietic reconstitution, but different genes play different roles at various time, and cooperate with each other for hematopoietic reconstitution. |
| المشرفين على المادة: | U3P01618RT (Fluorouracil) |
| تواريخ الأحداث: | Date Created: 20150629 Date Completed: 20151124 Latest Revision: 20181023 |
| رمز التحديث: | 20231215 |
| DOI: | 10.7534/j.issn.1009-2137.2015.03.047 |
| PMID: | 26117048 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1009-2137 |
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| DOI: | 10.7534/j.issn.1009-2137.2015.03.047 |