دورية أكاديمية
Ultrasensitive microfluidic array for serum pro-inflammatory cytokines and C-reactive protein to assess oral mucositis risk in cancer patients.
| العنوان: | Ultrasensitive microfluidic array for serum pro-inflammatory cytokines and C-reactive protein to assess oral mucositis risk in cancer patients. |
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| المؤلفون: | Krause CE; Department of Chemistry, University of Connecticut, 55 North Eagleville Road, Storrs, CT, 06269, USA., Otieno BA, Bishop GW, Phadke G, Choquette L, Lalla RV, Peterson DE, Rusling JF |
| المصدر: | Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2015 Sep; Vol. 407 (23), pp. 7239-43. Date of Electronic Publication: 2015 Jul 05. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Heidelberg : Springer-Verlag, 2002- |
| مواضيع طبية MeSH: | C-Reactive Protein/*analysis , Cytokines/*blood , Immunomagnetic Separation/*instrumentation , Protein Array Analysis/*instrumentation , Stomatitis/*blood , Stomatitis/*diagnosis, Biomarkers/blood ; Equipment Design ; Equipment Failure Analysis ; Humans ; Inflammation Mediators/blood ; Lab-On-A-Chip Devices ; Reproducibility of Results ; Sensitivity and Specificity |
| مستخلص: | In addition to disease diagnostics, there is a need for biomarkers to predict severity of cancer therapy side effects such as oral mucositis. Oral mucositis is an inflammatory lesion of oral mucosa caused by high-dose chemotherapy and/or radiation that is especially prevalent during oral cancer treatment. We describe here a semi-automated, modular microfluidic immunoarray optimized for ultrasensitive detection of pro-inflammatory cytokines involved in pathobiology of oral mucositis. Our goal is to methodologically identify risk of mucositis early in oral cancer treatment, before the onset of lesions. Biomarkers include tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and C-reactive protein (CRP). Protein analytes were captured from serum in a capture chamber by 1-μm magnetic beads coated with antibodies and enzyme labels. Beads are then transported downstream to a detection chamber containing an eight-sensor array coated with glutathione-coated gold nanoparticles (GSH-AuNP) and a second set of antibodies to capture the beads with analyte proteins. In this first application of the immunoarray to four-protein multiplexed measurements, ultralow detection limits of 10-40 fg mL(-1) in 5 μL serum were achieved for simultaneous detection in 30 min. Mass detection limits were 2.5-10 zmol, as few as 1500 molecules. Accuracy and diagnostic utility of the arrays were demonstrated by correlation of levels of the four biomarker proteins in serum from head and neck cancer patients with results from standard ELISA. This approach may lead to rapid, low-cost estimates of projected risk for severity of oral mucositis in cancer patients to enable improved therapeutic management. |
| References: | Int J Paediatr Dent. 2012 May;22(3):191-6. (PMID: 21919984) Oral Dis. 2015 Mar;21(2):133-41. (PMID: 24131518) Anal Chem. 2012 Jul 17;84(14):6249-55. (PMID: 22697359) Biosens Bioelectron. 2014 Mar 15;53:268-74. (PMID: 24144557) Pathol Oncol Res. 1997;3(2):126-129. (PMID: 11173639) Cancer Chemother Pharmacol. 2008 Jun;62(1):33-41. (PMID: 17703303) Head Neck. 2012 Jul;34(7):985-93. (PMID: 21953802) Nat Rev Cancer. 2004 Apr;4(4):277-84. (PMID: 15057287) Biosens Bioelectron. 2011 Jul 15;26(11):4477-83. (PMID: 21632234) Int J Radiat Oncol Biol Phys. 2009 Oct 1;75(2):393-8. (PMID: 19362778) Med Oral Patol Oral Cir Bucal. 2012 Jan;17(1):e10-5. (PMID: 21743397) |
| معلومات مُعتمدة: | R01 EB014586 United States EB NIBIB NIH HHS; K23DE016946 United States DE NIDCR NIH HHS; M01 RR006192 United States RR NCRR NIH HHS; EB014586 United States EB NIBIB NIH HHS; K23 DE016946 United States DE NIDCR NIH HHS; M01RR06192 United States RR NCRR NIH HHS |
| المشرفين على المادة: | 0 (Biomarkers) 0 (Cytokines) 0 (Inflammation Mediators) 9007-41-4 (C-Reactive Protein) |
| تواريخ الأحداث: | Date Created: 20150706 Date Completed: 20160523 Latest Revision: 20220318 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC4553140 |
| DOI: | 10.1007/s00216-015-8873-1 |
| PMID: | 26143063 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1618-2650 |
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| DOI: | 10.1007/s00216-015-8873-1 |