دورية أكاديمية
Detection of Low-Level Cardinium and Wolbachia Infections in Culicoides.
| العنوان: | Detection of Low-Level Cardinium and Wolbachia Infections in Culicoides. |
|---|---|
| المؤلفون: | Mee PT; Bio21 Institute and School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia CSIRO Health & Biosecurity, Australian Animal Health Laboratory, Geelong, Victoria, Australia., Weeks AR; Bio21 Institute and School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia., Walker PJ; CSIRO Health & Biosecurity, Australian Animal Health Laboratory, Geelong, Victoria, Australia., Hoffmann AA; Bio21 Institute and School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia., Duchemin JB; CSIRO Health & Biosecurity, Australian Animal Health Laboratory, Geelong, Victoria, Australia Jean-Bernard.Duchemin@csiro.au. |
| المصدر: | Applied and environmental microbiology [Appl Environ Microbiol] 2015 Sep; Vol. 81 (18), pp. 6177-88. Date of Electronic Publication: 2015 Jul 06. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5336 (Electronic) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Bacteroidetes/*isolation & purification , Ceratopogonidae/*microbiology , Wolbachia/*isolation & purification, Animals ; Australia ; Bacteroidetes/classification ; Bacteroidetes/genetics ; Cluster Analysis ; DNA Gyrase/genetics ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; DNA, Ribosomal/chemistry ; DNA, Ribosomal/genetics ; Molecular Sequence Data ; Phylogeny ; Polymerase Chain Reaction ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA ; Wolbachia/classification ; Wolbachia/genetics |
| مستخلص: | Bacterial endosymbionts have been identified as potentially useful biological control agents for a range of invertebrate vectors of disease. Previous studies of Culicoides (Diptera: Ceratopogonidae) species using conventional PCR assays have provided evidence of Wolbachia (1/33) and Cardinium (8/33) infections. Here, we screened 20 species of Culicoides for Wolbachia and Cardinium, utilizing a combination of conventional PCR and more sensitive quantitative PCR (qPCR) assays. Low levels of Cardinium DNA were detected in females of all but one of the Culicoides species screened, and low levels of Wolbachia were detected in females of 9 of the 20 Culicoides species. Sequence analysis based on partial 16S rRNA gene and gyrB sequences identified "Candidatus Cardinium hertigii" from group C, which has previously been identified in Culicoides from Japan, Israel, and the United Kingdom. Wolbachia strains detected in this study showed 98 to 99% sequence identity to Wolbachia previously detected from Culicoides based on the 16S rRNA gene, whereas a strain with a novel wsp sequence was identified in Culicoides narrabeenensis. Cardinium isolates grouped to geographical regions independent of the host Culicoides species, suggesting possible geographical barriers to Cardinium movement. Screening also identified Asaia bacteria in Culicoides. These findings point to a diversity of low-level endosymbiont infections in Culicoides, providing candidates for further characterization and highlighting the widespread occurrence of these endosymbionts in this insect group. (Copyright © 2015, American Society for Microbiology. All Rights Reserved.) |
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| المشرفين على المادة: | 0 (DNA, Bacterial) 0 (DNA, Ribosomal) 0 (RNA, Ribosomal, 16S) EC 5.99.1.3 (DNA Gyrase) |
| تواريخ الأحداث: | Date Created: 20150708 Date Completed: 20160516 Latest Revision: 20220408 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC4542234 |
| DOI: | 10.1128/AEM.01239-15 |
| PMID: | 26150447 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5336 |
|---|---|
| DOI: | 10.1128/AEM.01239-15 |