دورية أكاديمية
Simultaneous determination of hyzetimibe and its main active metabolite in plasma by LC-MS/MS and its application in PK study.
| العنوان: | Simultaneous determination of hyzetimibe and its main active metabolite in plasma by LC-MS/MS and its application in PK study. |
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| المؤلفون: | Chen J; Center of Clinical Pharmacology, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China., Lou H; Center of Clinical Pharmacology, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China., Jiang B; Center of Clinical Pharmacology, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China., Shao R; Center of Clinical Pharmacology, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China., Ruan Z; Center of Clinical Pharmacology, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China., Wang J; Center of Clinical Pharmacology, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. |
| المصدر: | Bioanalysis [Bioanalysis] 2015; Vol. 7 (15), pp. 1857-67. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Future Science Country of Publication: England NLM ID: 101512484 Publication Model: Print Cited Medium: Internet ISSN: 1757-6199 (Electronic) Linking ISSN: 17576180 NLM ISO Abbreviation: Bioanalysis Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Future Science, 2009- |
| مواضيع طبية MeSH: | Anticholesteremic Agents/*chemistry , Azetines/*chemistry , Chromatography, Liquid/*methods , Fluorobenzenes/*chemistry , Tandem Mass Spectrometry/*methods, Anticholesteremic Agents/metabolism ; Azetines/metabolism ; Fluorobenzenes/metabolism ; Humans |
| مستخلص: | Background: Hyzetimibe is a new compound belonging to a novel class of selective cholesterol absorption inhibitors. A simple, highly sensitive LC-MS/MS method has been developed for the quantification of hyzetimibe and its main active metabolite, hyzetimibe-glucuronide, in human plasma. Results: Analytical samples were prepared using a protein precipitation method coupled with a concentration process. The linearity of this method was established for concentrations in the ranges of 0.05-50 and 0.5-500 ng/ml for hyzetimibe and hyzetimibe-glucuronide, respectively. The accuracy and precision of the method varied from 97.9 to 105% and 2.6 to 7.4%, respectively. Conclusion: This study represents the first reported example of an LC-MS/MS assay for the simultaneous quantification of hyzetimibe and its main active metabolite, hyzetimibe-glucuronide, in human plasma. Furthermore, this method has been successfully applied to a PK study. |
| المشرفين على المادة: | 0 (1-(4-fluorophenyl)-3-(3-(4-fluorophenyl)-4-hydroxybut-2-enyl)-4-(4-hydroxyphenyl)-2-azetidinone) 0 (Anticholesteremic Agents) 0 (Azetines) 0 (Fluorobenzenes) |
| تواريخ الأحداث: | Date Created: 20150822 Date Completed: 20160518 Latest Revision: 20150822 |
| رمز التحديث: | 20221213 |
| DOI: | 10.4155/bio.15.114 |
| PMID: | 26295987 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1757-6199 |
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| DOI: | 10.4155/bio.15.114 |