دورية أكاديمية
Modulation of violacein production and phenotypes associated with biofilm by exogenous quorum sensing N-acylhomoserine lactones in the marine bacterium Pseudoalteromonas ulvae TC14.
| العنوان: | Modulation of violacein production and phenotypes associated with biofilm by exogenous quorum sensing N-acylhomoserine lactones in the marine bacterium Pseudoalteromonas ulvae TC14. |
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| المؤلفون: | Mireille Ayé A; Université de Toulon, MAPIEM, EA 4323, 83957 La Garde, France., Bonnin-Jusserand M; Université de Toulon, MAPIEM, EA 4323, 83957 La Garde, France.; Unité Biochimie des Produits Aquatiques (BPA), Université du Littoral Côte d'Opale, Institut Charles Viollette, EA 7394, 62327 Boulogne sur Mer, France., Brian-Jaisson F; Université de Toulon, MAPIEM, EA 4323, 83957 La Garde, France., Ortalo-Magné A; Université de Toulon, MAPIEM, EA 4323, 83957 La Garde, France., Culioli G; Université de Toulon, MAPIEM, EA 4323, 83957 La Garde, France., Koffi Nevry R; Laboratoire de Microbiologie et Biotechnologie, Université Nangui-Abrogoua 02 BP 801, Abidjan 02, Côte d'Ivoire., Rabah N; Université de Toulon, MAPIEM, EA 4323, 83957 La Garde, France., Blache Y; Université de Toulon, MAPIEM, EA 4323, 83957 La Garde, France., Molmeret M; Université de Toulon, MAPIEM, EA 4323, 83957 La Garde, France. |
| المصدر: | Microbiology (Reading, England) [Microbiology (Reading)] 2015 Oct; Vol. 161 (10), pp. 2039-2051. Date of Electronic Publication: 2015 Aug 28. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 9430468 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1465-2080 (Electronic) Linking ISSN: 13500872 NLM ISO Abbreviation: Microbiology (Reading) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: Reading, U.K. : Society for General Microbiology, c1994- |
| مواضيع طبية MeSH: | Quorum Sensing*, Acyl-Butyrolactones/*metabolism , Biofilms/*growth & development , Indoles/*metabolism , Pigments, Biological/*metabolism , Pseudoalteromonas/*drug effects , Pseudoalteromonas/*physiology, Aquatic Organisms/drug effects ; Aquatic Organisms/physiology ; Bacterial Adhesion ; Locomotion ; Mediterranean Sea |
| مستخلص: | Various phenotypes ranging from biofilm formation to pigment production have been shown to be regulated by quorum sensing (QS) in many bacteria. However, studies of the regulation of pigments produced by marine bacteria in saline conditions and of biofilm-associated phenotypes are scarcer. This study focuses on the demonstration of the existence of a QS communication system involving N-acylhomoserine lactones (AHLs) in the Mediterranean Sea strain Pseudoalteromonas ulvae TC14. We have investigated whether TC14 produces the violacein pigment, and whether intrinsic or exogenous AHLs could influence its production and modulate biofilm-associated phenotypes. Here, we demonstrate that the purple pigment produced by TC14 is violacein. The study shows that in planktonic conditions, TC14 produces more pigment in the medium in which it grows less. Using different approaches, the results also show that TC14 does not produce intrinsic AHLs in our conditions. When exogenous AHLs are added in planktonic conditions, the production of violacein is upregulated by C6-, C12-, 3-oxo-C8 and 3-oxo-C12-HSLs (homoserine lactones), and downregulated by 3-oxo-C6-HSL. In sessile conditions, 3-oxo-C8-HSL upregulates the production of violacein. The study of the biofilm-associated phenotypes shows that oxo-derived-HSLs decrease adhesion, swimming and biofilm formation. While 3-oxo-C8 and 3-oxo-C12-HSLs decrease both swimming and adhesion, 3-oxo-C6-HSLs decrease not only violacein production in planktonic conditions but also swimming, adhesion and more subtly biofilm formation. Therefore, TC14 may possess a functional LuxR-type QS receptor capable of sensing extrinsic AHLs, which controls violacein production, motility, adhesion and biofilm formation. |
| المشرفين على المادة: | 0 (Acyl-Butyrolactones) 0 (Indoles) 0 (Pigments, Biological) QJH0DSQ3SG (violacein) |
| تواريخ الأحداث: | Date Created: 20150831 Date Completed: 20160725 Latest Revision: 20200826 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1099/mic.0.000147 |
| PMID: | 26318530 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1465-2080 |
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| DOI: | 10.1099/mic.0.000147 |