دورية أكاديمية
Enhanced cytotoxicity of photoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells.
| العنوان: | Enhanced cytotoxicity of photoexcited fullerene C60 and cisplatin combination against drug-resistant leukemic cells. |
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| المؤلفون: | Franskevych DV, Prylutska SV; Taras Shevchenko National University of Kyiv, Kyiv 01601, Ukraine., Grynyuk II; Taras Shevchenko National University of Kyiv, Kyiv 01601, Ukraine., Grebinyk DM; Taras Shevchenko National University of Kyiv, Kyiv 01601, Ukraine., Matyshevska OP; Taras Shevchenko National University of Kyiv, Kyiv 01601, Ukraine. |
| المصدر: | Experimental oncology [Exp Oncol] 2015 Sep; Vol. 37 (3), pp. 187-91. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Akademperiodyka Country of Publication: Ukraine NLM ID: 101230541 Publication Model: Print Cited Medium: Internet ISSN: 2312-8852 (Electronic) Linking ISSN: 18129269 NLM ISO Abbreviation: Exp Oncol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: [Kyiv] : Akademperiodyka Original Publication: Kyiv : Morion, [2004]- |
| مواضيع طبية MeSH: | Drug Resistance, Neoplasm* , Fullerenes*/chemistry, Cisplatin/*administration & dosage , Cisplatin/*toxicity, Animals ; Cell Line, Tumor ; Cell Survival/drug effects ; Dose-Response Relationship, Drug ; Humans ; Leukemia/metabolism ; Mice ; Reactive Oxygen Species/metabolism |
| مستخلص: | Aim: To evaluate the viability of leukemic cells sensitive (L1210S) and resistant (L1210R) to cisplatin, ROS production and free cytosolic Ca(2+) concentration under treatment with cisplatin or its combination with photoexcited fullerene C60. Methods: Cell viability was assessed by the MTT reduction assay. Light-emitting diode lamp (2.45 J/cm(2)) was used for photoexcitation of intracellular accumulated fullerene C60. Free cytosolic calcium concentration ([Ca(2+)]i) and ROS production in cells were estimated with the use of fluorescent probes Indo-1 and 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA), respectively. Results: It is shown that viability of L1210R cells wasn't changed under treatment with cisplatin in concentration range 0.1-10 μg/ml. 50% and 30% decrease of L1210S cells were observed after 24 h of incubation with cisplatin at concentrations 5 and 1 μg/ml, respectively. Intensification of extranuclear cytotoxic effects (ROS production and [Ca(2+)]i increase) after treatment with 1 μg/ml was detected in L1210S, but not in L1210R cells. The most strongly pronounced increase of ROS production and [Ca(2+)]i in both L1210 cell lines was revealed in dynamics after combined treatment with cisplatin (1 μg/ml) and photoexcited fullerene C60 (10(-5) M) and was followed by decreased viability of not only L1210S, but of L1210R cells as well.. Conclusion: Combined treatment with photoexcited C60 and cisplatin allowed to decrease effective concentration of cisplatin against parental L1210 cells and to increase sensibility of resistant cells to the drug. |
| المشرفين على المادة: | 0 (Fullerenes) 0 (Reactive Oxygen Species) NP9U26B839 (fullerene C60) Q20Q21Q62J (Cisplatin) |
| تواريخ الأحداث: | Date Created: 20151001 Date Completed: 20160413 Latest Revision: 20220330 |
| رمز التحديث: | 20231215 |
| PMID: | 26422102 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2312-8852 |
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