دورية أكاديمية
أعرض في EDS

Quantitative proteomic analysis of Araucaria angustifolia (Bertol.) Kuntze cell lines with contrasting embryogenic potential.

التفاصيل البيبلوغرافية
العنوان: Quantitative proteomic analysis of Araucaria angustifolia (Bertol.) Kuntze cell lines with contrasting embryogenic potential.
المؤلفون: dos Santos AL; Department of Botany, Institute of Bioscience, University of São Paulo, São Paulo, São Paulo, Brazil., Elbl P; Department of Botany, Institute of Bioscience, University of São Paulo, São Paulo, São Paulo, Brazil., Navarro BV; Department of Botany, Institute of Bioscience, University of São Paulo, São Paulo, São Paulo, Brazil., de Oliveira LF; Department of Botany, Institute of Bioscience, University of São Paulo, São Paulo, São Paulo, Brazil., Salvato F; Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, São Paulo, Brazil., Balbuena TS; College of Agricultural and Veterinary Sciences, São Paulo State University, Jaboticabal, São Paulo, Brazil., Floh EI; Department of Botany, Institute of Bioscience, University of São Paulo, São Paulo, São Paulo, Brazil. Electronic address: enyfloh@usp.br.
المصدر: Journal of proteomics [J Proteomics] 2016 Jan 01; Vol. 130, pp. 180-9. Date of Electronic Publication: 2015 Oct 03.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101475056 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-7737 (Electronic) Linking ISSN: 18743919 NLM ISO Abbreviation: J Proteomics Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier
مواضيع طبية MeSH: Plant Proteins/*metabolism , Proteome/*metabolism , Proteomics/*methods , Tracheophyta/*metabolism, Carbohydrate Metabolism ; Carbohydrates/chemistry ; Databases, Protein ; Electrophoresis, Gel, Two-Dimensional ; Gene Expression Regulation ; Genotype ; Plant Somatic Embryogenesis Techniques ; RNA/chemistry ; Reactive Oxygen Species/metabolism ; Seeds/metabolism ; Sequence Analysis, RNA ; Tandem Mass Spectrometry ; Trypsin/chemistry
مستخلص: GeLC–MS/MS based label free proteomic profiling was used in the large scale identification and quantification of proteins from Brazilian pine (Araucaria angustifolia) embryogenic cell (EC) lines that showed different propensities to form somatic embryos. Using a predicted protein sequence database that was derived from A. angustifolia RNA-Seq data, 2398 non-redundant proteins were identified. The log2 of the spectral count values of 858 proteins of these proteins showed a normal distribution, and were used for statistical analysis. Statistical tests indicated that 106 proteins were significantly differentially abundant between the two EC lines, and that 35 were more abundant in the responsive genotype (EC line SE1) and 71 were more abundant in the blocked genotype (EC line SE6). An increase in the abundance of proteins related to cell defense, anti-oxidative stress responses, and storage reserve deposition was observed in SE1. Moreover, in SE6 we observed an increased abundance of two proteins associated with seed development during the embryogenic cell proliferation stage, which we suggest is associated with genotypes showing a low responsiveness to embryo formation. Differences in protein abundance between the EC lines are discussed in terms of carbohydrate metabolism, cell division, defense response, gene expression, and response to reactive oxygen species.
فهرسة مساهمة: Keywords: Conifer; Embryogenic potential; Molecular markers; Quantitative proteomics; Somatic embryogenesis
المشرفين على المادة: 0 (Carbohydrates)
0 (Plant Proteins)
0 (Proteome)
0 (Reactive Oxygen Species)
63231-63-0 (RNA)
EC 3.4.21.4 (Trypsin)
تواريخ الأحداث: Date Created: 20151006 Date Completed: 20160830 Latest Revision: 20191210
رمز التحديث: 20240628
DOI: 10.1016/j.jprot.2015.09.027
PMID: 26435419
قاعدة البيانات: MEDLINE
الوصف
تدمد:1876-7737
DOI:10.1016/j.jprot.2015.09.027