دورية أكاديمية
Purification and molecular characterization of a novel mannose-specific lectin from Dioclea reflexa hook seeds with inflammatory activity.
| العنوان: | Purification and molecular characterization of a novel mannose-specific lectin from Dioclea reflexa hook seeds with inflammatory activity. |
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| المؤلفون: | Pinto-Junior VR; Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Correia JL; Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Pereira RI; Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Pereira-Junior FN; Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Santiago MQ; Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Osterne VJ; Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Madeira JC; Laboratório de Fisio-Farmacologia da Inflamação (LAFFIN), Institute of Biomedical Sciences, State University of Ceará, Fortaleza, CE, Brazil., Cajazeiras JB; Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Nagano CS; Laboratório de Espectrometria de Massas aplicado a Proteínas (LEMAP), Federal University of Ceará, Fortaleza, CE, Brazil., Delatorre P; Laboratório de Biologia Molecular Estrutural e Oncogenética (LBMEO), Department of Molecular Biology, Federal University of Paraíba, João Pessoa, PB, Brazil., Assreuy AM; Laboratório de Fisio-Farmacologia da Inflamação (LAFFIN), Institute of Biomedical Sciences, State University of Ceará, Fortaleza, CE, Brazil., Nascimento KS; Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Cavada BS; Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil. |
| المصدر: | Journal of molecular recognition : JMR [J Mol Recognit] 2016 Apr; Vol. 29 (4), pp. 134-41. Date of Electronic Publication: 2015 Oct 14. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Country of Publication: England NLM ID: 9004580 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1099-1352 (Electronic) Linking ISSN: 09523499 NLM ISO Abbreviation: J Mol Recognit Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Original Publication: London, UK : Heydon & Son, c1988- |
| مواضيع طبية MeSH: | Dioclea/*chemistry , Edema/*chemically induced , Mannose/*pharmacology , Plant Lectins/*isolation & purification , Plant Lectins/*pharmacology, Animals ; Chromatography, Affinity ; Erythrocytes/drug effects ; Hemagglutination/drug effects ; Inflammation Mediators/isolation & purification ; Inflammation Mediators/pharmacology ; Mice ; Plant Lectins/chemistry ; Protein Structure, Secondary ; Rabbits |
| مستخلص: | A novel lectin present in Dioclea reflexa seeds (DrfL) was discovered and described in this study. DrfL was purified in a single step by affinity chromatography in a Sephadex G-50 column. The lectin strongly agglutinated rabbit erythrocytes and was inhibited by α-methyl-D-mannoside, D-mannose, and D-glucose. The hemagglutinating activity of DrfL is optimum at pH 5.0-7.0, stable up to 50 °C, and dependent on divalent cations. Similar to other lectins of the subtribe Diocleinae, the analysis by mass spectrometry indicated that DrfL has three chains (α, β, and γ) with masses of 25,562, 12,874, and 12,706 Da, respectively, with no disulfide bonds or glycosylation. DrfL showed inflammatory activity in the paw edema model and exhibited low cytotoxicity against Artemia sp. (Copyright © 2015 John Wiley & Sons, Ltd.) |
| فهرسة مساهمة: | Keywords: Dioclea reflexa; ESI mass spectrometry; inflammatory activity; lectin |
| المشرفين على المادة: | 0 (Inflammation Mediators) 0 (Plant Lectins) PHA4727WTP (Mannose) |
| تواريخ الأحداث: | Date Created: 20151015 Date Completed: 20161213 Latest Revision: 20161230 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1002/jmr.2512 |
| PMID: | 26464029 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1099-1352 |
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| DOI: | 10.1002/jmr.2512 |