دورية أكاديمية
Virus evolution during chronic hepatitis B virus infection as revealed by ultradeep sequencing data.
| العنوان: | Virus evolution during chronic hepatitis B virus infection as revealed by ultradeep sequencing data. |
|---|---|
| المؤلفون: | Jones LR; Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, 9 de Julio y Begrano S/N (9100) Trelew, Chubut, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas, Av. Rivadavia 1917 (C1083ACA) Buenos Aires, Argentina., Sede M; Consejo Nacional de Investigaciones Científicas y Técnicas, Av. Rivadavia 1917 (C1083ACA) Buenos Aires, Argentina.; Instituto de Investigaciones Biomédicas en Retrovirus y Sida (INBIRS), Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155-Piso 11 (C1121ABG) Buenos Aires, Argentina., Manrique JM; Consejo Nacional de Investigaciones Científicas y Técnicas, Av. Rivadavia 1917 (C1083ACA) Buenos Aires, Argentina.; Laboratorio de Virología y Genética Molecular, Facultad de Ciencias Naturales sede Trelew, Universidad Nacional de la Patagonia San Juan Bosco, 9 de Julio y Begrano S/N (9100) Trelew, Chubut, Argentina., Quarleri J; Instituto de Investigaciones Biomédicas en Retrovirus y Sida (INBIRS), Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155-Piso 11 (C1121ABG) Buenos Aires, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas, Av. Rivadavia 1917 (C1083ACA) Buenos Aires, Argentina. |
| المصدر: | The Journal of general virology [J Gen Virol] 2016 Feb; Vol. 97 (2), pp. 435-444. Date of Electronic Publication: 2015 Nov 18. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 0077340 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1465-2099 (Electronic) Linking ISSN: 00221317 NLM ISO Abbreviation: J Gen Virol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: London, Cambridge Univ. Press for the Society for General Microbiology. |
| مواضيع طبية MeSH: | Evolution, Molecular* , Genetic Variation* , High-Throughput Nucleotide Sequencing*, Hepatitis B virus/*classification , Hepatitis B virus/*genetics , Hepatitis B, Chronic/*virology, Child ; Cluster Analysis ; DNA, Viral/chemistry ; DNA, Viral/genetics ; Family Health ; Female ; Hepatitis B virus/isolation & purification ; Humans ; Infant ; Male ; Molecular Sequence Data ; Phylogeny ; Sequence Analysis, DNA ; Sequence Homology |
| مستخلص: | Despite chronic hepatitis B virus (HBV) infection (CHB) being a leading cause of liver cirrhosis and cancer, HBV evolution during CHB is not fully understood. Recent studies have indicated that virus diversity progressively increases along the course of CHB and that some virus mutations correlate with severe liver conditions such as chronic hepatitis, cirrhosis and hepatocellular carcinoma. Using ultradeep sequencing (UDS) data from an intrafamilial case, we detected such mutations at low frequencies among three immunotolerant patients and at high frequencies in an inactive carrier. Furthermore, our analyses indicated that the HBV population from the seroconverter patient underwent many genetic changes in response to virus clearance. Together, these data indicate a potential use of UDS for developing non-invasive biomarkers for monitoring disease changes over time or in response to specific therapies. In addition, our analyses revealed that virus clearance seemed not to require the virus effective population size to decline. A detailed genetic analysis of the viral lineages arising during and after the clearance suggested that mutations at or close to critical elements of the core promoter (enhancer II, epsilon encapsidation signal, TA2, TA3 and direct repeat 1-hormone response element) might be responsible for a sustained replication. This hypothesis requires the decline in virus load to be explained by constant clearance of virus-producing hepatocytes, consistent with the sustained progress towards serious liver conditions experienced by many CHB patients. |
| المشرفين على المادة: | 0 (DNA, Viral) |
| تواريخ الأحداث: | Date Created: 20151120 Date Completed: 20160627 Latest Revision: 20191209 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1099/jgv.0.000344 |
| PMID: | 26581478 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1465-2099 |
|---|---|
| DOI: | 10.1099/jgv.0.000344 |