دورية أكاديمية
The Multitarget Ligand 3-Iodothyronamine Modulates β-Adrenergic Receptor 2 Signaling.
| العنوان: | The Multitarget Ligand 3-Iodothyronamine Modulates β-Adrenergic Receptor 2 Signaling. |
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| المؤلفون: | Dinter J; Institut für Experimentelle Pädiatrische Endokrinologie, Berlin, Germany., Khajavi N; Department of Ophthalmology, Charité-Universitätsmedizin Berlin, Berlin, Germany., Mühlhaus J; Institut für Experimentelle Pädiatrische Endokrinologie, Berlin, Germany., Wienchol CL; Institut für Experimentelle Pädiatrische Endokrinologie, Berlin, Germany., Cöster M; Institut für Biochemie, Molekulare Biochemie, Medizinische Fakultät, University of Leipzig, Leipzig, Germany., Hermsdorf T; Institut für Biochemie, Molekulare Biochemie, Medizinische Fakultät, University of Leipzig, Leipzig, Germany., Stäubert C; Institut für Biochemie, Molekulare Biochemie, Medizinische Fakultät, University of Leipzig, Leipzig, Germany., Köhrle J; Institut für Experimentelle Endokrinologie, Charité-Universitätsmedizin Berlin, Berlin, Germany., Schöneberg T; Institut für Biochemie, Molekulare Biochemie, Medizinische Fakultät, University of Leipzig, Leipzig, Germany., Kleinau G; Institut für Experimentelle Pädiatrische Endokrinologie, Berlin, Germany., Mergler S; Department of Ophthalmology, Charité-Universitätsmedizin Berlin, Berlin, Germany., Biebermann H; Institut für Experimentelle Pädiatrische Endokrinologie, Berlin, Germany. |
| المصدر: | European thyroid journal [Eur Thyroid J] 2015 Sep; Vol. 4 (Suppl 1), pp. 21-9. Date of Electronic Publication: 2015 May 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bioscientifica Ltd Country of Publication: England NLM ID: 101604579 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2235-0640 (Print) Linking ISSN: 22350640 NLM ISO Abbreviation: Eur Thyroid J Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: 2022- : [Bristol] : Bioscientifica Ltd. Original Publication: Basel : S. Karger |
| مستخلص: | Background: 3-Iodothyronamine (3-T1AM), a signaling molecule with structural similarities to thyroid hormones, induces numerous physiological responses including reversible body temperature decline. One target of 3-T1AM is the trace amine-associated receptor 1 (TAAR1), which is a member of the rhodopsin-like family of G protein-coupled receptors (GPCRs). Interestingly, the effects of 3-T1AM remain detectable in TAAR1 knockout mice, suggesting further targets for 3-T1AM such as adrenergic receptors. Therefore, we evaluated whether β-adrenergic receptor 1 (ADRB1) and 2 (ADRB2) signaling is affected by 3-T1AM in HEK293 cells and in human conjunctival epithelial cells (IOBA-NHC), where these receptors are highly expressed endogenously. Methods: A label-free EPIC system for prescreening the 3-T1AM-induced effects on ADRB1 and ADRB2 in transfected HEK293 cells was used. In addition, ADRB1 and ADRB2 activation was analyzed using a cyclic AMP assay and a MAPK reporter gene assay. Finally, fluorescence Ca(2+) imaging was utilized to delineate 3-T1AM-induced Ca(2+) signaling. Results: 3-T1AM (10(-5)-10(-10)M) enhanced isoprenaline-induced ADRB2-mediated Gs signaling but not that of ADRB1-mediated signaling. MAPK signaling remained unaffected for both receptors. In IOBA-NHC cells, norepinephrine-induced Ca(2+) influxes were blocked by the nonselective ADRB blocker timolol (10 µM), indicating that ADRBs are most likely linked with Ca(2+) channels. Notably, timolol was also found to block 3-T1AM (10(-5)M)-induced Ca(2+) influx. Conclusions: The presented data support that 3-T1AM directly modulates β-adrenergic receptor signaling. The relationship between 3-T1AM and β-adrenergic signaling also reveals a potential therapeutic value for suppressing Ca(2+) channel-mediated inflammation processes, occurring in eye diseases such as conjunctivitis. |
| References: | Cell Physiol Biochem. 2014;34(3):790-803. (PMID: 25170901) Int J Mol Sci. 2014 Nov 10;15(11):20638-55. (PMID: 25391046) Pharmacol Rev. 2010 Dec;62(4):701-25. (PMID: 21079041) Pharmacol Rev. 2015;67(1):36-73. (PMID: 25361914) Invest Ophthalmol Vis Sci. 2011 Jan 21;52(1):485-93. (PMID: 20739465) Biochem J. 2008 May 15;412(1):103-12. (PMID: 18237275) Invest Ophthalmol Vis Sci. 2005 Feb;46(2):504-13. (PMID: 15671275) Histochem Cell Biol. 2012 Jun;137(6):743-61. (PMID: 22327830) Brain Res. 2010 Sep 10;1351:130-40. (PMID: 20615397) Cell Signal. 2015 Feb;27(2):315-25. (PMID: 25460045) Nat Med. 2004 Jun;10(6):638-42. (PMID: 15146179) Mol Cell Endocrinol. 2012 Apr 4;351(2):326-36. (PMID: 22269095) Endocrinology. 2009 Mar;150(3):1108-11. (PMID: 19116337) J Neurosci. 2010 Oct 27;30(43):14543-51. (PMID: 20980612) Front Physiol. 2014 Oct 16;5:402. (PMID: 25360120) Cell. 2008 Oct 31;135(3):561-71. (PMID: 18984166) Nat Med. 2010 Dec;16(12):1396-9. (PMID: 21076394) Cell Signal. 2007 Sep;19(9):1928-38. (PMID: 17561373) J Mol Endocrinol. 2013 Jun 29;51(1):109-18. (PMID: 23674133) Invest Ophthalmol Vis Sci. 2003 Oct;44(10):4263-74. (PMID: 14507870) Ophthalmic Res. 2014;52(3):151-9. (PMID: 25301091) Exp Eye Res. 2013 Nov;116:337-49. (PMID: 24135298) Ophthalmic Res. 1992;24(4):213-9. (PMID: 1359487) J Biol Chem. 1985 Mar 25;260(6):3440-50. (PMID: 3838314) Nat Biotechnol. 2010 Sep;28(9):943-9. (PMID: 20711173) Endocr Rev. 2011 Feb;32(1):64-80. (PMID: 20880963) Mol Pharmacol. 2000 Nov;58(5):954-66. (PMID: 11040042) J Cell Mol Med. 2009 Sep;13(9B):3082-90. (PMID: 19298522) Trends Pharmacol Sci. 2007 Aug;28(8):374-81. (PMID: 17629959) Biol Reprod. 1991 Feb;44(2):207-24. (PMID: 1849023) Invest Ophthalmol Vis Sci. 1978 Nov;17(11):1069-75. (PMID: 212382) Curr Eye Res. 2014 Nov;39(11):1076-80. (PMID: 24749907) J Biol Chem. 2010 Mar 26;285(13):9410-9. (PMID: 20110357) Endocr Res. 1984;10(2):91-9. (PMID: 6094171) Proc Natl Acad Sci U S A. 1988 Dec;85(24):9494-8. (PMID: 2849109) |
| فهرسة مساهمة: | Keywords: Calcium channel; Calcium homeostasis; Human conjunctiva; Signaling; Thyronamine; β-Adrenergic receptors |
| تواريخ الأحداث: | Date Created: 20151125 Date Completed: 20151125 Latest Revision: 20200930 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC4640289 |
| DOI: | 10.1159/000381801 |
| PMID: | 26601070 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2235-0640 |
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| DOI: | 10.1159/000381801 |