دورية أكاديمية
The vasorelaxant effect of gallic acid involves endothelium-dependent and -independent mechanisms.
| العنوان: | The vasorelaxant effect of gallic acid involves endothelium-dependent and -independent mechanisms. |
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| المؤلفون: | de Oliveira LM; Department of Pharmacology, Laboratory of Molecular and Biochemistry Pharmacology, Institute of Biological Sciences, Federal University of Goiás, Goiânia, GO, Brazil., de Oliveira TS; Department of Pharmacology, Laboratory of Molecular and Biochemistry Pharmacology, Institute of Biological Sciences, Federal University of Goiás, Goiânia, GO, Brazil., da Costa RM; Department of Pharmacology, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, SP, Brazil., de Souza Gil E; Faculty of Pharmacy, Federal University of Goiás, Goiânia, GO, Brazil., Costa EA; Department of Pharmacology, Laboratory of Pharmacology of Natural Products, Institute of Biological Sciences, Federal University of Goiás, Goiânia, GO, Brazil., Passaglia Rde C; Department of Pharmacology, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, SP, Brazil., Filgueira FP; Institute of Health Sciences, Federal University of Goias, Jatai, GO, Brazil., Ghedini PC; Department of Pharmacology, Laboratory of Molecular and Biochemistry Pharmacology, Institute of Biological Sciences, Federal University of Goiás, Goiânia, GO, Brazil. Electronic address: pcghedini@gmail.com. |
| المصدر: | Vascular pharmacology [Vascul Pharmacol] 2016 Jun; Vol. 81, pp. 69-74. Date of Electronic Publication: 2015 Nov 28. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 101130615 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3649 (Electronic) Linking ISSN: 15371891 NLM ISO Abbreviation: Vascul Pharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Elsevier Science, c2002- |
| مواضيع طبية MeSH: | Aorta, Thoracic/*drug effects , Endothelium, Vascular/*drug effects , Gallic Acid/*pharmacology , Vasodilation/*drug effects , Vasodilator Agents/*pharmacology, Animals ; Aorta, Thoracic/metabolism ; Calcium Channel Blockers/pharmacology ; Calcium Channels, L-Type/drug effects ; Calcium Channels, L-Type/metabolism ; Dose-Response Relationship, Drug ; Endothelium, Vascular/metabolism ; Female ; In Vitro Techniques ; Nitric Oxide Synthase Type III/metabolism ; Phosphorylation ; Potassium Channels/agonists ; Potassium Channels/metabolism ; Rats, Wistar ; Signal Transduction/drug effects |
| مستخلص: | The mechanisms of action involved in the vasorelaxant effect of gallic acid (GA) were examined in the isolated rat thoracic aorta. GA exerted a relaxant effect in the highest concentrations (0.4-10mM) in both endothelium-intact and endothelium-denuded aortic rings. Pre-incubation with L-NAME, ODQ, calmidazolium, TEA, 4-aminopyridine, and barium chloride significantly reduced the pEC50 values. Moreover, this effect was not modified by indomethacin, wortmannin, PP2, glibenclamide, or paxillin. Pre-incubation of GA (1, 3, and 10mM) in a Ca(2+)-free Krebs solution attenuated CaCl2-induced contractions and blocked BAY K8644-induced vascular contractions, but it did not inhibit a contraction induced by the release of Ca(2+) from the sarcoplasmatic reticulum stores. In addition, a Western blot analysis showed that GA induces phosphorylation of eNOS in rat thoracic aorta. These results suggest that GA induces relaxation in rat aortic rings through an endothelium-dependent pathway, resulting in eNOS phosphorylation and opening potassium channels. Additionally, the relaxant effect by an endothelium-independent pathway involves the blockade of the Ca(2+) influx via L-type Ca(2+) channels. (Copyright © 2015 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Calcium channel; Gallic acid; Nitric oxide; Potassium channels; Rat thoracic aorta; Vasorelaxant |
| المشرفين على المادة: | 0 (Calcium Channel Blockers) 0 (Calcium Channels, L-Type) 0 (Potassium Channels) 0 (Vasodilator Agents) 632XD903SP (Gallic Acid) EC 1.14.13.39 (Nitric Oxide Synthase Type III) EC 1.14.13.39 (Nos3 protein, rat) |
| تواريخ الأحداث: | Date Created: 20151209 Date Completed: 20170605 Latest Revision: 20220408 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.vph.2015.10.010 |
| PMID: | 26643780 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1879-3649 |
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| DOI: | 10.1016/j.vph.2015.10.010 |