دورية أكاديمية
أعرض في EDS

Microbial metabolomics in open microscale platforms.

التفاصيل البيبلوغرافية
العنوان: Microbial metabolomics in open microscale platforms.
المؤلفون: Barkal LJ; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.; Carbone Cancer Center, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA., Theberge AB; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.; Carbone Cancer Center, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.; Department of Urology, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA., Guo CJ; Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles California 90089, USA., Spraker J; Department of Plant Pathology, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA., Rappert L; Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA., Berthier J; Department of Biotechnology, CEA-University Grenoble-Alpes, 17 Avenue des Martyrs, 38054 Grenoble, France., Brakke KA; Department of Mathematics, Susquehanna University, Selinsgrove, Pennsylvania 17870, USA., Wang CCC; Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles California 90089, USA.; Department of Chemistry, University of Southern California, Los Angeles California 90089, USA., Beebe DJ; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.; Carbone Cancer Center, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA., Keller NP; Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.; Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA., Berthier E; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.; Carbone Cancer Center, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.
المصدر: Nature communications [Nat Commun] 2016 Feb 04; Vol. 7, pp. 10610. Date of Electronic Publication: 2016 Feb 04.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Metabolome*, Aspergillus/*metabolism , Fusarium/*metabolism , Metabolomics/*methods , Pseudomonas aeruginosa/*metabolism , Ralstonia solanacearum/*metabolism, Aspergillus flavus/metabolism ; Aspergillus fumigatus/metabolism ; Aspergillus nidulans/metabolism ; Chromatography, High Pressure Liquid ; Coculture Techniques ; Culture Techniques ; Microfluidics ; Tandem Mass Spectrometry
مستخلص: The microbial secondary metabolome encompasses great synthetic diversity, empowering microbes to tune their chemical responses to changing microenvironments. Traditional metabolomics methods are ill-equipped to probe a wide variety of environments or environmental dynamics. Here we introduce a class of microscale culture platforms to analyse chemical diversity of fungal and bacterial secondary metabolomes. By leveraging stable biphasic interfaces to integrate microculture with small molecule isolation via liquid-liquid extraction, we enable metabolomics-scale analysis using mass spectrometry. This platform facilitates exploration of culture microenvironments (including rare media typically inaccessible using established methods), unusual organic solvents for metabolite isolation and microbial mutants. Utilizing Aspergillus, a fungal genus known for its rich secondary metabolism, we characterize the effects of culture geometry and growth matrix on secondary metabolism, highlighting the potential use of microscale systems to unlock unknown or cryptic secondary metabolites for natural products discovery. Finally, we demonstrate the potential for this class of microfluidic systems to study interkingdom communication between fungi and bacteria.
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معلومات مُعتمدة: K12 DK100022 United States DK NIDDK NIH HHS; 5T15LM007359 United States LM NLM NIH HHS; P30 CA014520 United States CA NCI NIH HHS; T32 GM008692 United States GM NIGMS NIH HHS; T15 LM007359 United States LM NLM NIH HHS; U24 DK097154 United States DK NIDDK NIH HHS; R01 AI065728 United States AI NIAID NIH HHS; T32 ES007015 United States ES NIEHS NIH HHS
تواريخ الأحداث: Date Created: 20160205 Date Completed: 20160627 Latest Revision: 20220410
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC4742997
DOI: 10.1038/ncomms10610
PMID: 26842393
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/ncomms10610