دورية أكاديمية
أعرض في EDS

High-density grids for efficient data collection from multiple crystals.

التفاصيل البيبلوغرافية
العنوان: High-density grids for efficient data collection from multiple crystals.
المؤلفون: Baxter EL; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Aguila L; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Alonso-Mori R; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Barnes CO; Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Bonagura CA; Art Robbins Instruments, Sunnyvale, CA 94089, USA., Brehmer W; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Brunger AT; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA., Calero G; Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Caradoc-Davies TT; The ARC Centre of Excellence in Advanced Molecular Imaging, Monash University, Melbourne, Victoria 3800, Australia., Chatterjee R; Physical Bioscences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Degrado WF; Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94158, USA., Fraser JS; Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA 94158, USA., Ibrahim M; Institut für Biologie, Humboldt-Universität zu Berlin, 10099 Berlin, Germany., Kern J; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Kobilka BK; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA., Kruse AC; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA., Larsson KM; Stanford University School of Medicine, Stanford, CA 94305, USA., Lemke HT; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Lyubimov AY; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA., Manglik A; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA., McPhillips SE; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Norgren E; Art Robbins Instruments, Sunnyvale, CA 94089, USA., Pang SS; The ARC Centre of Excellence in Advanced Molecular Imaging, Monash University, Melbourne, Victoria 3800, Australia., Soltis SM; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Song J; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Thomaston J; Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94158, USA., Tsai Y; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Weis WI; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA., Woldeyes RA; Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA 94158, USA., Yachandra V; Physical Bioscences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Yano J; Physical Bioscences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Zouni A; Institut für Biologie, Humboldt-Universität zu Berlin, 10099 Berlin, Germany., Cohen AE; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
المصدر: Acta crystallographica. Section D, Structural biology [Acta Crystallogr D Struct Biol] 2016 Jan; Vol. 72 (Pt 1), pp. 2-11. Date of Electronic Publication: 2016 Jan 01.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: John Wiley & Sons Country of Publication: United States NLM ID: 101676043 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2059-7983 (Electronic) Linking ISSN: 20597983 NLM ISO Abbreviation: Acta Crystallogr D Struct Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: <2018-> : Medford, MA : John Wiley & Sons
Original Publication: [Malden, MA] : John Wiley & Sons, [2016]-
مواضيع طبية MeSH: Crystallization/*instrumentation , Crystallography, X-Ray/*instrumentation, Animals ; Crystallization/economics ; Crystallization/methods ; Crystallography, X-Ray/economics ; Crystallography, X-Ray/methods ; Data Collection ; Diffusion ; Equipment Design ; Humans ; Temperature ; Volatilization
مستخلص: Higher throughput methods to mount and collect data from multiple small and radiation-sensitive crystals are important to support challenging structural investigations using microfocus synchrotron beamlines. Furthermore, efficient sample-delivery methods are essential to carry out productive femtosecond crystallography experiments at X-ray free-electron laser (XFEL) sources such as the Linac Coherent Light Source (LCLS). To address these needs, a high-density sample grid useful as a scaffold for both crystal growth and diffraction data collection has been developed and utilized for efficient goniometer-based sample delivery at synchrotron and XFEL sources. A single grid contains 75 mounting ports and fits inside an SSRL cassette or uni-puck storage container. The use of grids with an SSRL cassette expands the cassette capacity up to 7200 samples. Grids may also be covered with a polymer film or sleeve for efficient room-temperature data collection from multiple samples. New automated routines have been incorporated into the Blu-Ice/DCSS experimental control system to support grids, including semi-automated grid alignment, fully automated positioning of grid ports, rastering and automated data collection. Specialized tools have been developed to support crystallization experiments on grids, including a universal adaptor, which allows grids to be filled by commercial liquid-handling robots, as well as incubation chambers, which support vapor-diffusion and lipidic cubic phase crystallization experiments. Experiments in which crystals were loaded into grids or grown on grids using liquid-handling robots and incubation chambers are described. Crystals were screened at LCLS-XPP and SSRL BL12-2 at room temperature and cryogenic temperatures.
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معلومات مُعتمدة: R21 GM110580 United States GM NIGMS NIH HHS; GM055302 United States GM NIGMS NIH HHS; T32 GM008424 United States GM NIGMS NIH HHS; GM110501 United States GM NIGMS NIH HHS; R56 GM055302 United States GM NIGMS NIH HHS; T32 GM008294 United States GM NIGMS NIH HHS; NIH GM110580 United States GM NIGMS NIH HHS; R01 GM110501 United States GM NIGMS NIH HHS; P41GM103393 United States GM NIGMS NIH HHS; United States Howard Hughes Medical Institute; T32 GM064337 United States GM NIGMS NIH HHS; R01 GM112686 United States GM NIGMS NIH HHS; P41 GM103393 United States GM NIGMS NIH HHS; R01 GM055302 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: XFELs; automation for sample-exchange robots; high-throughput crystallography; sample delivery; serial crystallography
تواريخ الأحداث: Date Created: 20160220 Date Completed: 20161108 Latest Revision: 20231111
رمز التحديث: 20231111
مُعرف محوري في PubMed: PMC4756618
DOI: 10.1107/S2059798315020847
PMID: 26894529
قاعدة البيانات: MEDLINE
الوصف
تدمد:2059-7983
DOI:10.1107/S2059798315020847