دورية أكاديمية
أعرض في EDS

Phenotypic and genetic characterization of Piscirickettsia salmonis from Chilean and Canadian salmonids.

التفاصيل البيبلوغرافية
العنوان: Phenotypic and genetic characterization of Piscirickettsia salmonis from Chilean and Canadian salmonids.
المؤلفون: Otterlei A; Fish Diseases Research Group, Department of Biology, University of Bergen, P.O. 7803, N-5020, Bergen, Norway., Brevik ØJ; Cermaq Group AS, Dronning Eufemias gate 16, P.O. Box 144 Sentrum, N-0102, Oslo, Norway., Jensen D; Fish Diseases Research Group, Department of Biology, University of Bergen, P.O. 7803, N-5020, Bergen, Norway., Duesund H; Cermaq Group AS, Dronning Eufemias gate 16, P.O. Box 144 Sentrum, N-0102, Oslo, Norway., Sommerset I; MSD Animal Health, Thormølensgt. 55, 5008, Bergen, Norway., Frost P; MSD Animal Health, Thormølensgt. 55, 5008, Bergen, Norway., Mendoza J; Cermaq Chile, Diego Portales 2000, piso 10, Puerto Montt, Chile., McKenzie P; Cermaq Canada, 203 - 919 Island Highway, Campbell River, BC, V9W 2C2, Canada., Nylund A; Fish Diseases Research Group, Department of Biology, University of Bergen, P.O. 7803, N-5020, Bergen, Norway., Apablaza P; Cermaq Group AS, Dronning Eufemias gate 16, P.O. Box 144 Sentrum, N-0102, Oslo, Norway. Patricia.Apablaza@cermaq.com.
المصدر: BMC veterinary research [BMC Vet Res] 2016 Mar 15; Vol. 12, pp. 55. Date of Electronic Publication: 2016 Mar 15.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2005-
مواضيع طبية MeSH: Genetic Variation* , Phylogeny*, Piscirickettsia/*classification , Piscirickettsia/*physiology, Animals ; Anti-Bacterial Agents/pharmacology ; Canada ; Chile ; Culture Media ; Genotype ; Microbial Sensitivity Tests ; Oncorhynchus mykiss/microbiology ; Piscirickettsia/drug effects ; Piscirickettsia/genetics ; RNA, Ribosomal, 16S/genetics ; Temperature
مستخلص: Background: The study presents the phenotypic and genetic characterization of selected P. salmonis isolates from Atlantic salmon and rainbow trout suffering from SRS (salmonid rickettsial septicemia) in Chile and in Canada. The phenotypic characterization of the P. salmonis isolates were based on growth on different agar media (including a newly developed medium), different growth temperatures, antibiotics susceptibility and biochemical tests.
Results: This is the first study differentiating Chilean P. salmonis isolates into two separate genetic groups. Genotyping, based on 16S rRNA-ITS and concatenated housekeeping genes grouped the selected isolates into two clades, constituted by the Chilean strains, while the Canadian isolates form a branch in the phylogenetic tree. The latter consisted of two isolates that were different in both genetic and phenotypic characteristics. The phylogenies and the MLST do not reflect the origin of the isolates with respect to host species. The isolates included were heterogeneous in phenotypic tests.
Conclusions: The genotyping methods developed in this study provided a tool for separation of P. salmonis isolates into distinct clades. The SRS outbreaks in Chile are caused by minimum two different genetic groups of P. salmonis. This heterogeneity should be considered in future development of vaccines against this bacterium in Chile. Two different strains of P. salmonis, in regards to genetic and phenotypic characteristics, can occur in the same contemporary outbreak of SRS.
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فهرسة مساهمة: Keywords: 16S rDNA-ITS; Genotyping; Housekeeping genes; MLST; P. salmonis; Phenotyping; Phylogeny
المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (Culture Media)
0 (RNA, Ribosomal, 16S)
تواريخ الأحداث: Date Created: 20160316 Date Completed: 20161222 Latest Revision: 20181113
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC4791975
DOI: 10.1186/s12917-016-0681-0
PMID: 26975395
قاعدة البيانات: MEDLINE
الوصف
تدمد:1746-6148
DOI:10.1186/s12917-016-0681-0