دورية أكاديمية
Detection of a specific pattern of hyaluronan oligosaccharides and their binding proteins in human ovarian tumour.
| العنوان: | Detection of a specific pattern of hyaluronan oligosaccharides and their binding proteins in human ovarian tumour. |
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| المؤلفون: | Kolapalli SP; Department of Studies in Biochemistry, University of Mysore, Mysore, Karnataka, India.; Yenepoya Research Centre, Yenepoya University, Mangalore, Karnataka, India., Nunna V; Department of Studies in Biochemistry, University of Mysore, Mysore, Karnataka, India., Thomas A; Bharath Cancer Hospital, Mysore, Karnataka, India., Mortha KK; Department of Studies in Biochemistry, University of Mysore, Mysore, Karnataka, India., Banerjee SD; Department of Anatomy and Cellular Biology, Tufts University - School of Medicine, Boston, MA, USA., Boregowda RK; Rutgers Cancer Institute of New Jersey, Department of Medicine, Division of Medical Oncology - Rutgers, The State University of New Jersey, Robert Wood Johnson Medical School, New Brunswick, NJ, USA. |
| المصدر: | Cell biochemistry and function [Cell Biochem Funct] 2016 Jun; Vol. 34 (4), pp. 217-25. Date of Electronic Publication: 2016 Mar 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 8305874 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1099-0844 (Electronic) Linking ISSN: 02636484 NLM ISO Abbreviation: Cell Biochem Funct Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford, England : Wiley-Blackwell Original Publication: Guildford, Surrey : Butterworth Scientific Ltd., c1983- |
| مواضيع طبية MeSH: | Carrier Proteins/*metabolism , Hyaluronic Acid/*metabolism , Neoplasm Proteins/*metabolism , Oligosaccharides/*metabolism , Ovarian Neoplasms/*metabolism, Cell Membrane/metabolism ; Cell Nucleus/metabolism ; Female ; Humans ; Hyaluronan Receptors/metabolism ; Immunohistochemistry ; Molecular Weight ; Reference Standards |
| مستخلص: | Unlabelled: Tumour cells generate hyaluronan (HA) oligomers (O-HA) by an autocrine mechanism to regulate their own behaviour through receptor interaction, necessitating analysis of HA sizes and its receptor expression in tumour progression. In this study for the first time, we identified specific size of HA in malignant ovarian tumour compared to benign tumour tissue. Therefore, we prepared the identified HA probes and conducted multiplex and monoplex ligand blot analysis and Immunohistochemistry to identify their receptor expression and distribution. Although, HA recognized CD44 as principle receptors despite of size, multiplex analysis showed multiple receptor expression with distribution at the tumour cell surface. Furthermore, the HA 6-mer (major O-HA of ovarian tumour) pull down of tumour tissue proteins showed 120 kDa protein along with CD44 with over expression in the malignant tumour. Upon depletion of CD44 protein HA 6-mer showed a major 120 kDa protein with distribution at nuclear membrane, suggesting that this protein may play an important role in ovarian tumour progression. In summary, ovarian tumour cells of different grade showed heterogeneity in generation of HA oligomers and their interaction with specific receptors. Therefore, simultaneous analysis of O-HA and their receptors expression could serve as a prognostic indicator during tumorigenesis. Copyright © 2016 John Wiley & Sons, Ltd. Significance of the Study: Down regulation of hyaluronan (HA) at tumour epithelial cells results in the generation of O-HA. Increasing evidence about O-HA biological functions (mainly in vitro) are available, but we lack information of O-HA sizes generated during tumorigenesis and less information is available about their receptor interaction. We used biochemical approaches and identified that tumour cells of different grade possessing heterogeneity in generation of O-HA and specificity towards receptor binding. We identified a new receptor for HA 6-mer that over express in cancer tissue that shows its role in tumour progression. Collectively, simultaneous identification of HA sizes and their receptors could serve as a prognostic marker. (Copyright © 2016 John Wiley & Sons, Ltd.) |
| فهرسة مساهمة: | Keywords: CD44; HA 6-mers; HA oligosaccharides; hyaluronic acid; ovarian tumour |
| المشرفين على المادة: | 0 (CD44 protein, human) 0 (Carrier Proteins) 0 (Hyaluronan Receptors) 0 (Neoplasm Proteins) 0 (Oligosaccharides) 9004-61-9 (Hyaluronic Acid) |
| تواريخ الأحداث: | Date Created: 20160323 Date Completed: 20170123 Latest Revision: 20171116 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1002/cbf.3179 |
| PMID: | 27001487 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1099-0844 |
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| DOI: | 10.1002/cbf.3179 |